Anticorps Polyclonal de lapin anti-EIF3B

EIF3B Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 10319-1-AP

Synonymes

eIF 3 eta, EIF3 ETA, EIF3 P110, EIF3 P116, EIF3B, EIF3S9, hPrt1, PRT1, Prt1 homolog



Applications testées

Résultats positifs en WBcellules A375
Résultats positifs en IPcellules A375

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

10319-1-AP cible EIF3B dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène EIF3B Protéine recombinante Ag0386
Nom complet eukaryotic translation initiation factor 3, subunit B
Masse moléculaire calculée 93 kDa
Poids moléculaire observé 115 kDa
Numéro d’acquisition GenBankBC001173
Symbole du gène EIF3B
Identification du gène (NCBI) 8662
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EIF3B, also named as Eukaryotic translation initiation factor 3 subunit B, is a 814 amino acid protein, which contains 1 RRM (RNA recognition motif) domain and 8 WD repeats and belongs to the eIF-3 subunit B family. EIF3B as a RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. The calculated molecular weight of EIF3B is 93 kDa, but the phosphorylated EIF3B protein is about 115 kDa.

Protocole

Product Specific Protocols
WB protocol for EIF3B antibody 10319-1-APDownload protocol
IP protocol for EIF3B antibody 10319-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

SUMOylation of the m6A-RNA methyltransferase METTL3 modulates its function.

Authors - Yuzhang Du
humanWB

Elife

Translation initiation by the hepatitis C virus IRES requires eIF1A and ribosomal complex remodeling.

Authors - Zane A Jaafar
humanWB

Cancers (Basel)

GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells.

Authors - Chiara Bellio
mouseWB

bioRxiv

Novel gene-specific translation mechanism of dysregulated, chronic inflammation reveals promising, multifaceted COVID-19 therapeutics.

Authors - Li Wang
humanWB

Nat Commun

METTL3 acetylation impedes cancer metastasis via fine-tuning its nuclear and cytosolic functions

Authors - Yuanpei Li

Cell Chem Biol

Non-canonical function of histone methyltransferase G9a in the translational regulation of chronic inflammation

Authors - Adil Muneer