Anticorps Polyclonal de lapin anti-EIF2C1-Specific

EIF2C1-Specific Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 19690-1-AP

Synonymes

AGO1, Argonaute1, eIF 2C 1, EIF2C, eIF2C 1, EIF2C1, EIF2C1-Specific, GERP95, hAgo1, Protein argonaute 1, Q99

---

Galerie de données de validation

• 

  WB analysis of HeLa using 19690-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19690-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19690-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 19690-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19690-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19690-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 19690-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 19690-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 19690-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 19690-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 19690-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 19690-1-AP

  Immunofluorescent analysis of HepG2 cells using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HepG2 using 19690-1-AP

  Immunofluorescent analysis of HepG2 cells using 19690-1-AP (EIF2C1-Specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat, cellules K-562, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

19690-1-AP cible EIF2C1-Specific dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: eukaryotic translation initiation factor 2C, 1
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 97 kDa
• Numéro d’acquisition GenBank: NM_012199
• Symbole du gène: EIF2C1
• Identification du gène (NCBI): 26523
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EIF2C1, also named as AGO1, belongs to the argonaute family and Ago subfamily. EIF2C1 is required for RNA-mediated gene silencing (RNAi). It binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. EIF2C1 may also be required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). The antibody is specific to EIF2C1.

Publications

Species	Application	Title
human	IF	Front Mol Neurosci; miRNA Enriched in Human Neuroblast Nuclei Bind the MAZ Transcription Factor and Their Precursors Contain the MAZ Consensus Motif.; Authors - Belinda J Goldie
human	WB	Mol Ther Oncolytics; Hsa_circ_0001666 promotes non-small cell lung cancer migration and invasion through miR-1184/miR-548I/AGO1 axis.; Authors - Xueting Wang
mouse	IF	bioRxiv; MicroRNAs are necessary for the emergence of Purkinje cell identity; Authors - Norjin Zolboot

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Roy (Verified Customer) (06-15-2024)

Unfortunately it did detect both Ago1 and Ago2 (validation from siRNA experiments)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa