Anticorps Polyclonal de lapin anti-EIF2A/CDA02

• Phare
• Validé par KD/KO

EIF2A/CDA02 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

61

Avis (3)

N° de cat : 11233-1-AP

Synonymes

EIF2A, EIF2, EIF 2A, EIF, CDA02

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Galerie de données de validation

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  WB analysis using 11233-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11233-1-AP (EIF2A/CDA02 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11233-1-AP

  WB result of EIF2A/CDA02 antibody (11233-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2A/CDA02 transfected HeLa cells.

• 

  WB analysis using 11233-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11233-1-AP (EIF2A/CDA02 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11233-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11233-1-AP (EIF2A/CDA02 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 11233-1-AP

  IP result of anti-EIF2A/CDA02 (IP:11233-1-AP, 3ug; Detection:11233-1-AP 1:1000) with MCF-7 cells lysate 3250ug.

• 

  IP experiment of MCF-7 using 11233-1-AP

  IP result of anti-EIF2A/CDA02 (IP:11233-1-AP, 4ug; Detection:11233-1-AP 1:6000) with MCF-7 cells lysate 760 ug.

• 

  IHC staining of human prostate cancer using 11233-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11233-1-AP (EIF2A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 11233-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11233-1-AP (EIF2A/CDA02 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 11233-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11233-1-AP (EIF2A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 11233-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11233-1-AP (EIF2A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 11233-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11233-1-AP (EIF2A antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 11233-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF2A/CDA02 antibody (11233-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11233-1-AP

  Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 11233-1-AP (EIF2A antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

• 

  FC experiment of HepG2 using 11233-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug EIF2A/CDA02 Polyclonal antibody (11233-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HEK-293T, cellules HeLa, cellules PC-12
• Résultats positifs en IP: cellules MCF-7,
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HepG2 traitées à l'acide éthacrynique
• Résultats positifs en FC (Intra): cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 58 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

11233-1-AP cible EIF2A/CDA02 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF2A/CDA02 Protéine recombinante Ag1753
• Nom complet: eukaryotic translation initiation factor 2A, 65kDa
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC011885
• Symbole du gène: EIF2A
• Identification du gène (NCBI): 83939
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EIF2A, also named as CDA02, MSTP004 and MSTP089, belongs to the WD repeat EIF2A family. It functions in the early steps of protein synthesis of a small number of specific mRNAs. EIF2A, an alternative initiator tRNA-binding protein, has a key role in the translation of HCV mRNA during HCV infection, in turn promoting eIF2a phosphorylation by activating the eIF2a kinase PKR. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human EIF2A, recognizes specifically the 65kd EIF2A protein. EIF2A (Gene ID: 83939) and EIF2S1 (Gene ID: 1965) share the EIF2A symbol/alias in common. EIF2S1 is the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40S ribosomal subunit, the EIF2A protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP.

Publications

Species	Application	Title
	WB	Cell; Circadian clocks are modulated by compartmentalized oscillating translation; Authors - Yanrong Zhuang
mouse	WB	Nature; Translation from unconventional 5' start sites drives tumour initiation.; Authors - Ataman Sendoel ; KO Validated
	WB	Science; Translation from the 5' untranslated region shapes the integrated stress response.; Authors - Shelley R Starck ; KD Validated
human	WB	Science; Leucine-tRNA initiates at CUG start codons for protein synthesis and presentation by MHC class I.; Authors - Shelley R Starck ; KD Validated
human	WB	Nat Cell Biol; O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins; Authors - Yulin Chen
human	WB	Sci Adv; CReP mediates selective translation initiation at the endoplasmic reticulum.; Authors - Jonathan P Kastan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Gianluca (Verified Customer) (11-25-2021)

Good scientific feedback

• Applications: Western Blot

FH

Daniel (Verified Customer) (04-01-2019)

The provided antibody exceeded all expectations. It worked very well on the first try.Mouse liver was homogenized in Final sample buffer, and then proteins were separated by 10% SDS-PAGE followed by western blot with 11233-1-AP(EIF2A antibody) at a dilution of 1:1000 incubated at 4 degrees Celsius over night in 5% BSA TBST.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Liver

EIF2A/CDA02 Antibody Western Blot, validation (1:1000 dilution) in Mouse Liver (Cat no:11233-1-AP)

FH

Sahya (Verified Customer) (08-13-2018)

Showed a very strong signal, will use at a higher dilution next time.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Skeletal Muscle Satellite Cells