Anticorps Polyclonal de lapin anti-EHD3

• Phare
• Validé par KD/KO

EHD3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 25320-1-AP

Synonymes

EH domain containing 3, EH domain containing protein 3, EHD2, EHD3, PAST homolog 3, PAST3

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Galerie de données de validation

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  WB analysis using 25320-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25320-1-AP (EHD3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human peripheral blood platelets using 25320-1-AP

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 25320-1-AP (EHD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 25320-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25320-1-AP (EHD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 25320-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25320-1-AP (EHD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse lung using 25320-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 25320-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 25320-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25320-1-AP (EHD3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 25320-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25320-1-AP (EHD3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 25320-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 25320-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25320-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25320-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, plaquettes de sang périphérique humain, tissu cérébral de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu pulmonaire de souris, tissu cérébral de souris, tissu cérébral humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

25320-1-AP cible EHD3 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EHD3 Protéine recombinante Ag18216
• Nom complet: EH-domain containing 3
• Masse moléculaire calculée: 535 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC156996
• Symbole du gène: EHD3
• Identification du gène (NCBI): 30845
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
zebrafish	WB	Nat Cell Biol; Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.; Authors - Quanlong Lu ; KD Validated
mouse, zebrafish	WB,IF	Nat Cell Biol; Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.; Authors - Quanlong Lu ; KD Validated
human	WB	EBioMedicine; EHD1 impairs decidualization by regulating the Wnt4/β-catenin signaling pathway in recurrent implantation failure.; Authors - Quan Zhou
human	WB	EMBO J; Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.; Authors - Tatsuo Miyamoto ; KO Validated
rat	IHC	Int J Mol Sci; Proteomic Analysis of Dysfunctional Liver Sinusoidal Endothelial Cells Reveals Substantial Differences in Most Common Experimental Models of Chronic Liver Diseases; Authors - Mar Gil
mouse	IHC	Am J Physiol Renal Physiol; Unique miRNome and transcriptome profiles underlie microvascular heterogeneity in mouse kidney; Authors - Matthijs Luxen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kyosuke (Verified Customer) (06-12-2019)

I works well on HUVECs for Western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HUVECs