Anticorps Polyclonal de lapin anti-EFEMP2

EFEMP2 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 12004-1-AP

Synonymes

Fibulin-4, Fibulin 4, FIBL-4, FIBL 4, FBLN4

---

Galerie de données de validation

• 

  WB analysis of mouse kidney using 12004-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12004-1-AP (EFEMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12004-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12004-1-AP (EFEMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 12004-1-AP

  IP result of anti-EFEMP2 (IP:12004-1-AP, 4ug; Detection:12004-1-AP 1:1000) with mouse kidney tissue lysate 2160 ug.

• 

  IHC staining of human heart using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50.

• 

  IHC staining of human skin using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50.

• 

  IHC staining of human skin using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50.

• 

  IHC staining of human prostate cancer using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules K-562
• Résultats positifs en IP: tissu rénal de souris,
• Résultats positifs en IHC: tissu cardiaque humain, tissu cutané humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

12004-1-AP cible EFEMP2 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EFEMP2 Protéine recombinante Ag2640
• Nom complet: EGF-containing fibulin-like extracellular matrix protein 2
• Masse moléculaire calculée: 443 aa, 49 kDa
• Poids moléculaire observé: 52-55 kDa
• Numéro d’acquisition GenBank: BC010456
• Symbole du gène: EFEMP2
• Identification du gène (NCBI): 30008
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EFEMP2, also known as Fibulin-4 is a 60 kDa extracellular matrix (ECM) glycoprotein that is a pivotal oncogene in tumor progression and metastasis. EFEMP2 is necessary for elastic fiber formation and connective tissue development. Defects in this gene are cause of an autosomal recessive cutis laxa syndrome.

Publications

Species	Application	Title
human	WB	Cancer Biol Ther; STEAP2 promotes osteosarcoma progression by inducing epithelial–mesenchymal transition via the PI3K/AKT/mTOR signaling pathway and is regulated by EFEMP2; Authors - Dong Zhang
human	WB	Cell Mol Biol Lett; EFEMP2 upregulates PD-L1 expression via EGFR/ERK1/2/c-Jun signaling to promote the invasion of ovarian cancer cells; Authors - Xin Shen