Anticorps Polyclonal de lapin anti-EDC3

EDC3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 16486-1-AP

Synonymes

EDC3, hYjeF_N2, LSM16, LSM16 homolog, YJDC, YjeF_N2, YJEFN2

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Galerie de données de validation

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  WB analysis using 16486-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16486-1-AP (EDC3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16486-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16486-1-AP (EDC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16486-1-AP

  IP result of anti-EDC3 (IP:16486-1-AP, 4ug; Detection:16486-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human kidney using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16486-1-AP (EDC3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16486-1-AP (EDC3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 16486-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EDC3 antibody (16486-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules MCF-7
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque humain, tissu cutané humain, tissu hépatique humain, tissu placentaire humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

16486-1-AP cible EDC3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EDC3 Protéine recombinante Ag9608
• Nom complet: enhancer of mRNA decapping 3 homolog (S. cerevisiae)
• Masse moléculaire calculée: 508 aa, 56 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC021271
• Symbole du gène: EDC3
• Identification du gène (NCBI): 80153
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IF	Cancer Res; Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition; Authors - Liang Fang
mouse	IF	PLoS Genet; Two RNA binding proteins, ADAD2 and RNF17, interact to form a heterogeneous population of novel meiotic germ cell granules with developmentally dependent organelle association; Authors - Lauren G Chukrallah
rat	WB	Cell Rep; Identification of dynamic RNA-binding proteins uncovers a Cpeb4-controlled regulatory cascade during pathological cell growth of cardiomyocytes.; Authors - Eva Riechert
human		Nucleic Acids Res; RNA helicase DDX6 and scaffold protein GW182 in P-bodies promote biogenesis of stress granules; Authors - Vladimir Majerciak
human	IF,WB	J Cell Biol; DDX6 modulates P-body and stress granule assembly, composition, and docking; Authors - Nina Ripin