Anticorps Polyclonal de lapin anti-EAF1

EAF1 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 13787-1-AP

Synonymes

EAF1, ELL associated factor 1

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Galerie de données de validation

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  WB analysis of K-562 using 13787-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13787-1-AP (EAF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13787-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13787-1-AP (EAF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13787-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13787-1-AP (EAF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13787-1-AP

  IP result of anti-EAF1 (IP:13787-1-AP, 4ug; Detection:13787-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IF Staining of SH-SY5Y using 13787-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 13787-1-AP (EAF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

13787-1-AP cible EAF1 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EAF1 Protéine recombinante Ag4804
• Nom complet: ELL associated factor 1
• Masse moléculaire calculée: 268 aa, 29 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC041329
• Symbole du gène: EAF1
• Identification du gène (NCBI): 85403
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ELL associated factor 1 and ELL associated factor 2 (EAF1/2 factors) are reported to play important roles in tumor suppression and embryogenesis.The tumor suppressor EAF2 is regulated by androgen signaling and associated with prostate cancer. Eaf factors have been revealed to modulate mesoderm and neural patterning by inhibiting canonical Wnt signaling, and high activity of canonical Wnt/β-catenin is revealed in eafs morphants (PMID: 23364330).Catalog#13787-1-AP is a rabbit polyclonal antibody raised against the full-length of human EAF1.

Publications

Species	Application	Title
human	WB	Mol Cell; HIV-1 Tat assembles a multifunctional transcription elongation complex and stably associates with the 7SK snRNP.; Authors - Bijan Sobhian
human	WB	Cell Death Differ; Global identification of phospho-dependent SCF substrates reveals a FBXO22 phosphodegron and an ERK-FBXO22-BAG3 axis in tumorigenesis.; Authors - Ping Liu
	WB	Biochim Biophys Acta; Transcriptional factors Eaf1/2 inhibit endoderm and mesoderm formation via suppressing TGF-β signaling.; Authors - Jing-Xia Liu
human	WB	Biochim Biophys Acta Gene Regul Mech; ELL-associated factors EAF1/2 negatively regulate HIV-1 transcription through inhibition of Super Elongation Complex formation.; Authors - Rongdiao Liu
mouse	WB,IP	Cell; Inherited blood cancer predisposition through altered transcription elongation; Authors - Jiawei Zhao