Anticorps Polyclonal de lapin anti-DYRK1B

DYRK1B Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 12240-1-AP

Synonymes

DYRK1B, Minibrain related kinase, MIRK, Mirk protein kinase

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Galerie de données de validation

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  WB analysis of mouse testis using 12240-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 12240-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 12240-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 12240-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12240-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12240-1-AP (DYRK1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules HeLa, tissu cardiaque humain, tissu de côlon humain, tissu de muscle squelettique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

12240-1-AP cible DYRK1B dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DYRK1B Protéine recombinante Ag2883
• Nom complet: dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B
• Masse moléculaire calculée: 629 aa, 69 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC018751
• Symbole du gène: DYRK1B
• Identification du gène (NCBI): 9149
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DYRK1B(dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B) is also named as MIRK. It is a member of the dyrk/minibrain family of serine/threonine kinases that mediate the transition from growth to differentiation in lower eukaryotes and mammals(PMID:15546868). DYRK1B functions as a transcriptional coactivator that is activated by co-expressed MKK3, a MAP kinase that also can activate p38 MAP kinase(PMID:14500717). The DYRK1B splice variants found in each muscle tissue exhibit approximate molecular weights of 70 and 75 kDa(PMID:12902328). It has 3 isoforms produced by alternative splicing of the molecular weight of 65-69 kDa.

Publications

Species	Application	Title
human	WB	Am J Physiol Cell Physiol; MicroRNA-9 enhances the transactivation of nuclear factor of activated T cells by targeting KPNB1 and DYRK1B.; Authors - Yan Zeng