Anticorps Polyclonal de lapin anti-DTX3

• Phare
• Validé par KD/KO

DTX3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 25304-1-AP

Synonymes

deltex homolog 3 (Drosophila), Deltex3, DTX3, Protein deltex 3, RING finger protein 154, RNF154

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Galerie de données de validation

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  WB analysis using 25304-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25304-1-AP (DTX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of hTERT-RPE1 using 25304-1-AP

  Western blot analysis in wildtype and DTX3 knockouted human hTERT-RPE1 cells with 25304-1-AP (DTX3 antibody). The data provided by an anonymous customer.

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  WB analysis using 25304-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25304-1-AP (DTX3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 25304-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25304-1-AP (DTX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 25304-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25304-1-AP (DTX3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25304-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25304-1-AP (DTX3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 25304-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25304-1-AP (DTX3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 25304-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25304-1-AP (DTX3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 25304-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25304-1-AP (DTX3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 25304-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25304-1-AP (DTX3 Antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules hTERT-RPE1, tissu cérébral de souris, tissu ovarien de souris, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu rénal humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below

Informations sur le produit

25304-1-AP cible DTX3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DTX3 Protéine recombinante Ag18111
• Nom complet: deltex homolog 3 (Drosophila)
• Masse moléculaire calculée: 347 aa, 38 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC114498
• Symbole du gène: DTX3
• Identification du gène (NCBI): 196403
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DTX3, also named as Deltex3 or RING finger protein 154, is 347 amino acid protein, which contains one RING-type zinc finger and belongs to the Deltex family. DTX3 is a regulator of Notch signaling, which is involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations.

Publications

Species	Application	Title
human	WB	J Cancer; Deltex3 inhibits Epithelial Mesenchymal Transition in Papillary Thyroid Carcinoma via promoting ubiquitination of XRCC5 to regulate the AKT signal pathway.; Authors - Lidong Wang ; KD Validated
human	WB	Mol Biol Rep; Deltex E3 ubiquitin ligase 3 inhibits colorectal cancer cell growth and regulates cell cycle progression via upregulating E2F transcription factor 1.; Authors - Hongli Xu ; KO Validated