Anticorps Polyclonal de lapin anti-DSG1

DSG1 Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 24587-1-AP

Synonymes

Cadherin family member 4, CDHF4, desmoglein 1, Desmosomal glycoprotein 1, DG1, DGI, DSG, DSG1, Pemphigus foliaceus antigen, PPKS1, SPPK1

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Galerie de données de validation

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  IHC staining of human skin cancer using 24587-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:400 (under 10x lens). heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

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  IHC staining of human skin cancer using 24587-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

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  IHC staining of mouse skin using 24587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 24587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 24587-1-AP

  Immunofluorescent analysis of A431 cells using 24587-1-AP (DSG1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu de cancer de la peau humain, tissu cutané de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 7 publications below

Informations sur le produit

24587-1-AP cible DSG1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DSG1 Protéine recombinante Ag20184
• Nom complet: desmoglein 1
• Masse moléculaire calculée: 1049 aa, 114 kDa
• Numéro d’acquisition GenBank: BC153001
• Symbole du gène: DSG1
• Identification du gène (NCBI): 1828
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG1 is a single-pass transmembrane glycoprotein that is highly expressed in the epidermis and localized primarily within the suprabasal epithelial layers (PMID: 16286477; 24220297). DSG1 mediates intercellular adhesion and has a crucial role in maintaining epidermal integrity and barrier function (PMID: 23974871). It is also involved in epithelial cell differentiation (PMID: 23524961). Mutations in the DSG1 gene can cause the autosomal dominant disorder striate palmoplantar keratoderma and a syndrome featuring severe dermatitis, multiple allergies, and metabolic wasting (SAM syndrome) (PMID: 29315490; 23974871).

Publications

Species	Application	Title
human	IF	EMBO Rep; O-glycan initiation directs distinct biological pathways and controls epithelial differentiation.; Authors - Ieva Bagdonaite
human	IF	Stem Cell Res Ther; Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.; Authors - Ling Leng
	IF	J Cell Sci; Novel stress granules-like structures are induced via a paracrine mechanism during viral infection.; Authors - Valentina Iadevaia
mouse	WB,IF	Microscopy (Oxf); Inhibition of retinoid X receptor improved the morphology, localization of desmosomal proteins and paracellular permeability in three-dimensional cultures of mouse keratinocytes.; Authors - Shoko Ishikawa
mouse	IF	Stem Cell Reports; Organoids from mouse molar and incisor as new tools to study tooth-specific biology and development; Authors - Florian Hermans
mouse	IF,WB	Histochem Cell Biol; JNK inhibition enhances cell-cell adhesion impaired by desmoglein 3 gene disruption in keratinocytes; Authors - Shuhei Ogawa