Anticorps Polyclonal de lapin anti-CRMP4

CRMP4 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 18969-1-AP

Synonymes

CRMP 4, CRMP4, dihydropyrimidinase like 3, DPYSL3, DRP 3, DRP3, LCRMP, ULIP, ULIP 1, ULIP1, Unc 33 like phosphoprotein 1

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 18969-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18969-1-AP (CRMP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 18969-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18969-1-AP (CRMP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 18969-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18969-1-AP (CRMP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 18969-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 18969-1-AP (CRMP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 18969-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18969-1-AP (CRMP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 18969-1-AP

  IP result of anti-CRMP4 (IP:18969-1-AP, 3ug; Detection:18969-1-AP 1:700) with SH-SY5Y cells lysate 3000ug.

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  IHC staining of human liver cancer using 18969-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18969-1-AP (CRMP4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18969-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18969-1-AP (CRMP4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18969-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18969-1-AP (CRMP4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 18969-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18969-1-AP (CRMP4 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 18969-1-AP

  Immunofluorescent analysis of HepG2 cells, using DPYSL3 antibody 18969-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules Jurkat, tissu de cervelet de souris, tissu de thymus de souris
• Résultats positifs en IP: cellules SH-SY5Y
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

18969-1-AP cible CRMP4 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CRMP4 Protéine recombinante Ag5299
• Nom complet: dihydropyrimidinase-like 3
• Masse moléculaire calculée: 570 aa, 62 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC039006
• Symbole du gène: CRMP4
• Identification du gène (NCBI): 1809
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DPYSL3(Dihydropyrimidinase-related protein 3) is also named as CRMP4, DRP3, ULIP, ULIP1 and belongs to the hydantoinase/dihydropyrimidinase subfamily.DPYSL3 is a developmentally regulated protein, strongly expressed in early embryonic post-mitotic neural cells, reaching a peak of expression around the first post-natal week. In the adult brain, DPYSL3 is present mainly in regions that retain neurogenesis.DPYSL3 protein is also found in synaptic sites at the adult neuromuscular junction where it may be involved in the maintenance of neuronal stability(PMID: 16135096).It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Free Radic Biol Med; Proteomic analysis of the glutathione-deficient LEGSKO mouse lens reveals activation of EMT signaling, loss of lens specific markers, and changes in stress response proteins.; Authors - Jeremy A Whitson
rat	WB, IP	Biochem Biophys Res Commun; Up-regulation of DRP-3 long isoform during the induction of neural progenitor cells by glutamate treatment in the ex vivo rat retina.; Authors - Kazuhiro Tokuda