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Anticorps Polyclonal de lapin anti-DPP9

DPP9 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 11568-1-AP

Synonymes

dipeptidyl peptidase 9, Dipeptidyl peptidase IX, DP9, DPLP9, DPP IX, DPP9, DPRP 2, DPRP2

Galerie de données de validation

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  • Western Blot (WB) analysis of HepG2 cells using DPP9 Polyclonal antibody (11568-1-AP)

    WB analysis of HepG2 using 11568-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11568-1-AP (DPP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using DPP9 Polyclonal antibody (11568-1-AP)

    WB analysis of PC-3 using 11568-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11568-1-AP (DPP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using DPP9 Polyclonal antibody (11568-1-AP)

    WB analysis of MCF-7 using 11568-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 11568-1-AP (DPP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using DPP9 Polyclonal antibody (11568-1-AP)

    WB analysis of mouse heart using 11568-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 11568-1-AP (DPP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A2780 cells using DPP9 Polyclonal antibody (11568-1-AP)

    WB analysis of A2780 using 11568-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 11568-1-AP (DPP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using DPP9 Polyclonal antibody (11568-1-AP)

    IHC staining of human ovary tumor using 11568-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11568-1-AP (DPP9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using DPP9 Polyclonal antibody (11568-1-AP)

    IHC staining of human ovary tumor using 11568-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11568-1-AP (DPP9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DPP9 Polyclonal antibody (11568-1-AP)

    IF Staining of HepG2 using 11568-1-AP

    Immunofluorescent analysis of HepG2 cells using 11568-1-AP (DPP9 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using DPP9 Polyclonal antibody (11568-1-AP)

    IF Staining of PC-3 using 11568-1-AP

    Immunofluorescent analysis of PC-3 cells using 11568-1-AP (DPP9 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using DPP9 Polyclonal antibody (11568-1-AP)

    IF Staining of MCF-7 using 11568-1-AP

    Immunofluorescent analysis of MCF-7 cells using 11568-1-AP (DPP9 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Applications testées

Résultats positifs en WBcellules HepG2, cellules A2780, cellules MCF-7, cellules PC-3, tissu cardiaque de souris
Résultats positifs en IHCtissu de tumeur ovarienne humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules HepG2, cellules MCF-7, cellules PC-3

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

11568-1-AP cible DPP9 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène DPP9 Protéine recombinante Ag2145
Nom complet dipeptidyl-peptidase 9
Masse moléculaire calculée 863 aa, 98 kDa
Poids moléculaire observé 98 kDa
Numéro d’acquisition GenBankBC037948
Symbole du gène DPP9
Identification du gène (NCBI) 91039
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Dipeptidyl peptidase 9(DPP9), is a member of the S9B family in clan SC of the serine proteases. DPP9 has 3 isoforms with MW of 95, 98 and 101 kDa. DPP9 is involved in the regulation of the activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer.