Anticorps Polyclonal de lapin anti-DOCK8

• Phare
• Validé par KD/KO

DOCK8 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 11622-1-AP

Synonymes

dedicator of cytokinesis 8, DOCK8, MRD2, ZIR8

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Galerie de données de validation

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  WB analysis using 11622-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11622-1-AP (DOCK8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 11622-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11622-1-AP (DOCK8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 11622-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11622-1-AP (DOCK8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11622-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11622-1-AP (DOCK8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 11622-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11622-1-AP (DOCK8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 11622-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11622-1-AP (DOCK8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse lung using 11622-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11622-1-AP (DOCK8 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse lung using 11622-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11622-1-AP (DOCK8 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 11622-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11622-1-AP (DOCK8 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse kidney using 11622-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11622-1-AP (DOCK8 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules THP-1, tissu pulmonaire de rat
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque humain, tissu pulmonaire de souris, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

11622-1-AP cible DOCK8 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DOCK8 Protéine recombinante Ag2040
• Nom complet: dedicator of cytokinesis 8
• Masse moléculaire calculée: 2031 aa, 231 kDa
• Poids moléculaire observé: 230-239 kDa
• Numéro d’acquisition GenBank: BC019102
• Symbole du gène: DOCK8
• Identification du gène (NCBI): 81704
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Background

Dedicator of Cytokinesis 8 (DOCK8) is a protein that regulates the actin cytoskeleton, with particular importance in immune cells and a key role in innate and adaptive immune responses.

 What is the molecular weight of DOCK8?

231 kDa. DOCK8 is a protein composed of 2031 amino acids and is a guanine nucleotide exchange factor (GEF).

 What is the function of DOCK8?

DOCK8 is a member of the DOCK family of proteins, which have a unique DRH2 domain enabling them to act as GEFs and so controlling a range of cellular processes in various signaling pathways (PMID: 12432077). The specific target of DOCK8 is Cell division control protein 42 homolog (Cdc42), a small GTPase that is involved in regulation of the cell cycle and forms a complex. DOCK8 also acts as a scaffold molecule in this complex that initiates actin polymerization via the Wiskott-Aldrich Syndrome protein (WASp) (PubMed: 28028151, PubMed: 22461490).

 What diseases are associated with DOCK8?

The role of DOCK8 in immunity was first identified with the study of DOCK8-deficient patients who presented with combined immunodeficiency (PMID: 19776401; PMID: 20004785). The subsequent study of DOCK8 in immune cells such as T cells, natural killer (NK) cells, and B cells has revealed how it regulates their normal function. This includes the regulation of immune synapse formation, immune cell trafficking, regulation of dendritic cell polarization, and cytokine production (PMID: 28366940).

DOCK8 deficiency is caused by a number of different mutations in the gene. It leads to the autosomal recessive form of the immunodeficiency disease Hyper-IgE syndrome, or Job's syndrome. The symptoms of DOCK8 deficiency include eczema, high levels of serum IgE, hypereosinophilia, and recurrent respiratory and skin infections as a result of impaired immune cell function. 

Publications

Species	Application	Title
mouse	WB	Nat Immunol; Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions.; Authors - Rui Martins ; KO Validated
mouse	IF	Cell Death Differ; CCL2 regulation of MST1-mTOR-STAT1 signaling axis controls BCR signaling and B-cell differentiation.; Authors - Lu Yang
mouse	WB	Diabetes; Dedicator of Cytokinesis 5 Regulates Keratinocyte Function and Promotes Diabetic Wound Healing.; Authors - Hua Qu
human	WB, IF, IP	Oncotarget; CD147 promotes Src-dependent activation of Rac1 signaling through STAT3/DOCK8 during the motility of hepatocellular carcinoma cells.; Authors - Shi-Jie Wang ; KD Validated
human	WB	iScience; DOCK8-expressing T follicular helper cells newly generated beyond self-organized criticality cause systemic lupus erythematosus.; Authors - Shunichi Shiozawa ; KO Validated
mouse	WB	MedComm (2020); Ultra-high static magnetic fields cause immunosuppression through disrupting B-cell peripheral differentiation and negatively regulating BCR signaling; Authors - Heng Gu