Anticorps Polyclonal de lapin anti-DOCK3; MOCA

DOCK3; MOCA Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 20683-1-AP

Synonymes

dedicator of cytokinesis 3, DOCK3, DOCK3; MOCA, KIAA0299, MOCA, Modifier of cell adhesion, PBP, Presenilin binding protein

---

Galerie de données de validation

• 

  WB analysis using 20683-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 20683-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 20683-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 20683-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 20683-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20683-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20683-1-AP (DOCK3; MOCA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

20683-1-AP cible DOCK3; MOCA dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: dedicator of cytokinesis 3
• Masse moléculaire calculée: 233 kDa
• Poids moléculaire observé: 233 kDa
• Numéro d’acquisition GenBank: NM_004947
• Symbole du gène: DOCK3
• Identification du gène (NCBI): 1795
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Dedicator of cytokinesis 3 (DOCK3), also named as MOCA and PBP , is a ~180 kDa protein involved in signaling trasduction. It is a potential guanine nucleotide exchange factor (GEF) which activate some small GTPases by exchanging bound GDP for free GTP. DOCK3 is associated in Alzheimer disease tangles and regulates the accumulation of amyloid precursor protein and beta-amyloid. Overexpression of Dock3 in neural cells promotes axonal outgrowth downstream of brain-derived neurotrophic factor (BDNF) signaling. DOCK3 binds to and inactivates glycogen synthase kinase-3β (GSK-3β) at the plasma membrane, thereby promoteing axon branching and microtubule assembly. By stimulating actin polymerization and microtubule assembly, DOCK3 plays important roles downstream of BDNF signaling in the CNS.

Publications

Species	Application	Title
mouse	WB	Diabetes; Dedicator of Cytokinesis 5 Regulates Keratinocyte Function and Promotes Diabetic Wound Healing.; Authors - Hua Qu
human	WB	Int J Biochem Cell Biol; Inhibition of RAC1-GEF DOCK3 by miR-512-3p contributes to suppression of metastasis in non-small cell lung cancer.; Authors - Xingli Zhu