Anticorps Polyclonal de lapin anti-DOC2B

• Phare
• Validé par KD/KO

DOC2B Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 20574-1-AP

Synonymes

Doc2 beta, DOC2B, DOC2BL, double C2 like domains, beta

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Galerie de données de validation

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  WB analysis of HEK-293 using 20574-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20574-1-AP (DOC2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20574-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20574-1-AP (DOC2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20574-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20574-1-AP (DOC2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 20574-1-AP

  Immunofluorescent analysis of HepG2 cells, using DOC2B antibody 20574-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 20574-1-AP

  Immunofluorescent analysis of Hela cells, using DOC2B antibody 20574-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu cérébral de souris, tissu rénal de souris
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

20574-1-AP cible DOC2B dans les applications de WB, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: double C2-like domains, beta
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: NM_003585
• Symbole du gène: DOC2B
• Identification du gène (NCBI): 8447
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DOC2B may be involved in constitutive membrane trafficking including dynein-dependent intracellular vesicle transport. In vitro, binds calcium and phospholipids. The antibody is specific to DOC2B.

Publications

Species	Application	Title
mouse	WB,IF	Diabetes; Doc2b Protects β-Cells Against Inflammatory Damage and Enhances Function.; Authors - Arianne Aslamy ; KO Validated
human	WB	Cell Biol Toxicol; Metastatic suppression by DOC2B is mediated by inhibition of epithelial-mesenchymal transition and induction of senescence.; Authors - Samatha Bhat ; KD Validated
human	WB,CoIP	Pharmacol Res; DOC2B is a negative regulator of Wnt/β-catenin signaling pathway in cervical cancer.; Authors - Divya Adiga ; KD Validated
human	WB	J Biol Chem; DNA Promoter Methylation Dependent Transcription of Double C2 Like Domain Beta (DOC2B) Gene Regulates Tumor Growth in Human Cervical Cancer.; Authors - Shama Prasada Kabekkodu
human	WB,IF	Free Radic Biol Med; Double C-2 like domain beta (DOC2B) induces calcium dependent oxidative stress to promote lipotoxicity and mitochondrial dysfunction for its tumor suppressive function; Authors - Divya Adiga
mouse,rat	WB	Int J Mol Sci; A Novel Role for DOC2B in Ameliorating Palmitate-Induced Glucose Uptake Dysfunction in Skeletal Muscle Cells via a Mechanism Involving β-AR Agonism and Cofilin; Authors - Jinhee Hwang