Anticorps Polyclonal de lapin anti-DNMT2

• Phare
• Validé par KD/KO

DNMT2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 19221-1-AP

Synonymes

DNA MTase homolog HsaIIP, DNMT2, M.HsaIIP, PuMet, RNMT1, TRDMT1

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Galerie de données de validation

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  WB analysis of HeLa using 19221-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 19221-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19221-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 19221-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19221-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules MCF-7, tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

19221-1-AP cible DNMT2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNMT2 Protéine recombinante Ag5571
• Nom complet: tRNA aspartic acid methyltransferase 1
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 43-45 kDa
• Numéro d’acquisition GenBank: BC047733
• Symbole du gène: DNMT2
• Identification du gène (NCBI): 1787
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Dnmt2 was initially assigned a member of the DNA methyltransferase family on the basis of its extensive homology with eukaryotic and prokaryotic DNA-(cytosine C5)-methyltransferases . However, in the apparent absence of a phenotype in dnmt2 knockout cells, Dnmt2's possible biological function remained unknown , even though Dnmt2 is strongly conserved and it is found in species ranging from Schizosaccharomyces pombe to human. Later very weak, residual DNA methylation activity was found with enzymes from different species. The finding that Dnmt2 is an active RNA methyltransferase capable of methylating the C38 position of the tRNA Asp came as a surprise . However, still no cellular function of the tRNA Asp methylation has been found, although in Zebrafish Dnmt2 knock-down caused a developmental phenotype . It is very intriguing that an enzyme that looks like a DNA methyltransferase can methylate RNA, in particular since the RNA and DNA specific m5 C methyltransferases use different catalytic residues and a different mechanism for the methyl transfer reaction . the predicted molecular weight of Dnmt2 is 45 kDa, but it may migrate to the 40 kDa (PMID: 18567810 )

Publications

Species	Application	Title
human	WB	Clin Transl Med; Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.; Authors - Lijie Zhou
human	WB	BMC Biol; Multiple links between 5-methylcytosine content of mRNA and translation.; Authors - Ulrike Schumann ; KD Validated
human	WB,IF	Int J Biol Macromol; Restricted tRNA methylation by intermolecular disulfide bonds in DNMT2/TRDMT1; Authors - Huari Li
human	WB	Cell Death Dis; Epigenetic addition of m5C to HBV transcripts promotes viral replication and evasion of innate antiviral responses; Authors - Shuang Ding