Anticorps Polyclonal de lapin anti-DNASE2

• Phare
• Validé par KD/KO

DNASE2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 15934-1-AP

Synonymes

Acid DNase, Deoxyribonuclease 2 alpha, Deoxyribonuclease II alpha, DNase II alpha, DNASE2, DNASE2A, DNL, DNL2, Lysosomal DNase II, R31240_2

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Galerie de données de validation

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  WB analysis using 15934-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15934-1-AP (DNASE2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 15934-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15934-1-AP (DNASE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15934-1-AP

  WB result of DNASE2 antibody (15934-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNASE2 transfected HeLa cells.

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  IP experiment of U-87 MG using 15934-1-AP

  IP result of anti-DNASE2 (IP:15934-1-AP, 4ug; Detection:15934-1-AP 1:500) with U-87 MG cells lysate 1160 ug.

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  IP experiment of HeLa using 15934-1-AP

  IP result of anti-DNASE2 (IP:15934-1-AP, 4ug; Detection:15934-1-AP 1:500) with HeLa cells lysate 1360 ug.

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  IHC staining of mouse kidney using 15934-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15934-1-AP (DNASE2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 15934-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15934-1-AP (DNASE2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15934-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DNASE2 antibody (15934-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 15934-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DNASE2 antibody (15934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules LNCaP, cellules MDA-MB-231, cellules MG U-87
• Résultats positifs en IP: cellules MG U-87, cellules HeLa
• Résultats positifs en IHC: tissu rénal de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

15934-1-AP cible DNASE2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNASE2 Protéine recombinante Ag8781
• Nom complet: deoxyribonuclease II, lysosomal
• Masse moléculaire calculée: 360 aa, 40 kDa
• Poids moléculaire observé: 32-40 kDa
• Numéro d’acquisition GenBank: BC010419
• Symbole du gène: DNASE2
• Identification du gène (NCBI): 1777
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNASE2, or 'acid DNase,' hydrolyzes DNA under acidic conditions and is one of 2 distinct mammalian DNases. It hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. It has a key role in the degradation of nuclear DNA in cellular apoptosis during development [PMID:18812394]. Besieds, it is necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells. It has been demonstrated to be responsible forDNA degradation in apoptotic cells engulfed by macrophages and exogenous DNA on the skin surface [PMID:23019102]. DNASE2 exists some isoforms with MV 39 and 34 kDa. (PMID: 14644493)

Publications

Species	Application	Title
mouse	WB	J Biol Chem; Autolysosomal degradation of cytosolic chromatin fragments antagonizes oxidative stress-induced senescence.; Authors - Xiaojuan Han
human	WB	Pharmacol Res; Oroxylin A regulates cGAS DNA hypermethylation induced by methionine metabolism to promote HSC senescence; Authors - Danli Zhao
human,mouse	WB	Mol Nutr Food Res; Mangiferin protects DNase 2 abundance via Nrf2 activation to prevent cytosolic mtDNA accumulation during liver injury; Authors - Lisi Li