Anticorps Polyclonal de lapin anti-DNAJC10

• Phare
• Validé par KD/KO

DNAJC10 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 13101-1-AP

Synonymes

DNAJC10, ER resident protein ERdj5, ERdj5, JPDI, Macrothioredoxin, MTHr

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Galerie de données de validation

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  WB analysis using 13101-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13101-1-AP (DNAJC10 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13101-1-AP

  WB result of DNAJC10 antibody (13101-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJC10 transfected HeLa cells.

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  WB analysis of mouse liver using 13101-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13101-1-AP (DNAJC10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13101-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13101-1-AP (DNAJC10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13101-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13101-1-AP (DNAJC10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13101-1-AP

  IP result of anti-DNAJC10 (IP:13101-1-AP, 4ug; Detection:13101-1-AP 1:500) with HeLa cells lysate 1600 ug.

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  IHC staining of human pancreas cancer using 13101-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13101-1-AP (DNAJC10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 13101-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13101-1-AP (DNAJC10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 13101-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13101-1-AP (DNAJC10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 13101-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 13101-1-AP (DNAJC10 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach using 13101-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 13101-1-AP (DNAJC10 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 13101-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13101-1-AP (DNAJC10 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 13101-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13101-1-AP (DNAJC10 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 13101-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13101-1-AP (DNAJC10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13101-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13101-1-AP (DNAJC10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, tissu hépatique de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu d'estomac humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

13101-1-AP cible DNAJC10 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNAJC10 Protéine recombinante Ag3737
• Nom complet: DnaJ (Hsp40) homolog, subfamily C, member 10
• Masse moléculaire calculée: 793 aa, 91 kDa
• Poids moléculaire observé: 80-90 kDa
• Numéro d’acquisition GenBank: BC034713
• Symbole du gène: DNAJC10
• Identification du gène (NCBI): 54431
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNAJC10(DnaJ homolog subfamily C member 10)is also named as ERDJ5. The human ER-resident protein (ERdj5) ubiquitously expresses and is abundant in secretory tissues such as pancreas and testis and it may be involved in assisting protein folding and quality control in the ER(PMID:12411443). The deduced 793-amino acid protein has a calculated molecular mass of about 91 kD. ERDJ5 contains an N-terminal hydrophobic sequence, followed by a type III DnaJ domain, 4 thioredoxin-like domains, and a C-terminal KDEL endoplasmic reticulum (ER) retention signal. It can be modified by N-linked glycosylation(PMID:12446677). The ERdj5 antibody displays a higher affinity for endogenous ERdj5u.

Publications

Species	Application	Title
human	WB	PLoS Pathog; A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum.; Authors - Christopher Paul Walczak
human	WB,CoIP	Mol Cell Proteomics; Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis; Authors - Madison T. Wright
human	WB	Diabetes; Unbiased Profiling of the Human Proinsulin Biosynthetic Interaction Network Reveals a Role For Peroxiredoxin 4 in Proinsulin Folding.; Authors - Duc T Tran
human	WB	Mol Cell Proteomics; Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis.; Authors - Madison T Wright
human	WB	Mol Biol Cell; The ERdj5-Sel1L complex facilitates cholera toxin retrotranslocation.; Authors - Williams Jeffrey M JM
human	WB	Cell Stress Chaperones; Amyotrophic lateral sclerosis (ALS) and Alzheimer's disease (AD) are characterised by differential activation of ER stress pathways: focus on UPR target genes.; Authors - L Montibeller