Anticorps Polyclonal de lapin anti-DNAJB2
DNAJB2 Polyclonal Antibody for WB, IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IP, IF, IHC, ELISA
Conjugaison
Non conjugué
N° de cat : 10838-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | tissu cardiaque de souris, cellules A431, tissu cardiaque de rat, tissu cérébral de rat, tissu cérébral de souris, tissu de muscle squelettique de souris |
Résultats positifs en IP | tissu cérébral de souris |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 5 publications below |
IHC | See 2 publications below |
IF | See 2 publications below |
Informations sur le produit
10838-1-AP cible DNAJB2 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | DNAJB2 Protéine recombinante Ag1271 |
Nom complet | DnaJ (Hsp40) homolog, subfamily B, member 2 |
Masse moléculaire calculée | 36 kDa |
Poids moléculaire observé | 36-40 kDa |
Numéro d’acquisition GenBank | BC011609 |
Symbole du gène | DNAJB2 |
Identification du gène (NCBI) | 3300 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
The DNAJB2 (also known as HSJ1a, Heat-Shock Protein J1a) is a member of the DNAJ family of molecular chaperones which play important cellular roles in correct protein folding, in the response to protein misfolding, and in the degradation of misfolded proteins. It is expressed principally in the nervous system and is a key player in neuronal proteostasis maintenance. DNAJB2 has been proposed as a potent anti-aggregation chaperone for TDP-43, may play a role in the pathogenesis of ALS. DNAJB2 gene encodes two alternatively-spliced isoforms that differ in their C-terminus. The variant 1 form (V1 or HSJ1a) is expressed in both cytoplasm and nucleus, whereas the variant 2 form (V2 or HSJ1b) undergoes post-translational geranylgeranylation modification which mediates its attachment to the cytoplasmic side of the endoplasmic reticulum membrane. This antibody detects both isoforms of DNAJB2 with MW between 32-40 kDa. (PMID: 20395441)
Protocole
Product Specific Protocols | |
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WB protocol for DNAJB2 antibody 10838-1-AP | Download protocol |
IP protocol for DNAJB2 antibody 10838-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Ann Neurol A rare recessive distal hereditary motor neuropathy with HSJ1 chaperone mutation. | ||
Eur J Neurol DNAJB2-related Charcot-Marie-Tooth disease type 2: Pathomechanism insights and phenotypic spectrum widening. | ||
Sci Rep PolyQ-expanded proteins impair cellular proteostasis of ataxin-3 through sequestering the co-chaperone HSJ1 into aggregates. | ||
Cancers (Basel) Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin |