Anticorps Polyclonal de lapin anti-DNAJB2

DNAJB2 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 10838-1-AP

Synonymes

DnaJ protein homolog 1, DNAJB2, Heat shock 40 kDa protein 3, Heat shock protein J1, HSJ 1, HSJ1, HSPF3



Applications testées

Résultats positifs en WBtissu cardiaque de souris, cellules A431, tissu cardiaque de rat, tissu cérébral de rat, tissu cérébral de souris, tissu de muscle squelettique de souris
Résultats positifs en IPtissu cérébral de souris

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

10838-1-AP cible DNAJB2 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène DNAJB2 Protéine recombinante Ag1271
Nom complet DnaJ (Hsp40) homolog, subfamily B, member 2
Masse moléculaire calculée 36 kDa
Poids moléculaire observé 36-40 kDa
Numéro d’acquisition GenBankBC011609
Symbole du gène DNAJB2
Identification du gène (NCBI) 3300
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The DNAJB2 (also known as HSJ1a, Heat-Shock Protein J1a) is a member of the DNAJ family of molecular chaperones which play important cellular roles in correct protein folding, in the response to protein misfolding, and in the degradation of misfolded proteins. It is expressed principally in the nervous system and is a key player in neuronal proteostasis maintenance. DNAJB2 has been proposed as a potent anti-aggregation chaperone for TDP-43, may play a role in the pathogenesis of ALS. DNAJB2 gene encodes two alternatively-spliced isoforms that differ in their C-terminus. The variant 1 form (V1 or HSJ1a) is expressed in both cytoplasm and nucleus, whereas the variant 2 form (V2 or HSJ1b) undergoes post-translational geranylgeranylation modification which mediates its attachment to the cytoplasmic side of the endoplasmic reticulum membrane. This antibody detects both isoforms of DNAJB2 with MW between 32-40 kDa. (PMID: 20395441)

Protocole

Product Specific Protocols
WB protocol for DNAJB2 antibody 10838-1-APDownload protocol
IP protocol for DNAJB2 antibody 10838-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IF

Ann Neurol

A rare recessive distal hereditary motor neuropathy with HSJ1 chaperone mutation.

Authors - Blumen Sergiu C SC

Diabetes

PMID: 24379352

Authors - Brian G Drew
humanWB,IHC

Eur J Neurol

DNAJB2-related Charcot-Marie-Tooth disease type 2: Pathomechanism insights and phenotypic spectrum widening.

Authors - Paola Saveri
human,mouseWB,IHC

Am J Pathol

DNAJB2 expression in normal and diseased human and mouse skeletal muscle.

Authors - Claeys Kristl G KG
humanWB,IF

Sci Rep

PolyQ-expanded proteins impair cellular proteostasis of ataxin-3 through sequestering the co-chaperone HSJ1 into aggregates.

Authors - Hong-Wei Yue
humanWB

Cancers (Basel)

Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin

Authors - Mohamed Alalem