Anticorps Polyclonal de lapin anti-DNAJB12

• Phare
• Validé par KD/KO

DNAJB12 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 16780-1-AP

Synonymes

DJ10, DNAJB12

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Galerie de données de validation

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  WB analysis using 16780-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16780-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16780-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16780-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 16780-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16780-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 16780-1-AP

  IP Result of anti-DNAJB12 (IP:16780-1-AP, 4ug; Detection:16780-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A549, cellules MCF-7, cellules MKN-45, tissu cérébral de rat, tissu cérébral humain, tissu hépatique humain, tissu rénal humain
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• ChIP: See 1 publications below

Informations sur le produit

16780-1-AP cible DNAJB12 dans les applications de WB, IP, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, singe
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNAJB12 Protéine recombinante Ag10193
• Nom complet: DnaJ (Hsp40) homolog, subfamily B, member 12
• Masse moléculaire calculée: 375 aa, 42 kDa
• Poids moléculaire observé: 40-42 kDa
• Numéro d’acquisition GenBank: BC011812
• Symbole du gène: DNAJB12
• Identification du gène (NCBI): 54788
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,ChIP	Mol Cell; An oncogenic JMJD6-DGAT1 axis tunes the epigenetic regulation of lipid droplet formation in clear cell renal cell carcinoma.; Authors - Jin Zhou
human	IP	Nat Commun; The methyltransferase METTL9 mediates pervasive 1-methylhistidine modification in mammalian proteomes.; Authors - Erna Davydova
	WB, IP	J Cell Biol; Reticulon protects the integrity of the ER membrane during ER escape of large macromolecular protein complexes.; Authors - Yu-Jie Chen
human	WB	PLoS Pathog; A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum.; Authors - Christopher Paul Walczak
human	WB,IF	J Cell Biol; Reticulons promote formation of ER-derived double-membrane vesicles that facilitate SARS-CoV-2 replication; Authors - Jeffrey M Williams
human	IF	J Virol; The ER membrane J protein C18 executes a distinct role in promoting SV40 membrane penetration.; Authors - Parikshit Bagchi