Anticorps Polyclonal de lapin anti-DLX5

• Phare
• Validé par KD/KO

DLX5 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 10592-1-AP

Synonymes

distal less homeobox 5, DLX5, Homeobox protein DLX 5

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Galerie de données de validation

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  WB analysis of A549 using 10592-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10592-1-AP (DLX5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 10592-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10592-1-AP (DLX5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 10592-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 10592-1-AP (DLX5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human thymus using 10592-1-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 10592-1-AP (DLX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thymus using 10592-1-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 10592-1-AP (DLX5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules A375, cellules U-251
• Résultats positifs en IHC: tissu de thymus humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Informations sur le produit

10592-1-AP cible DLX5 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DLX5 Protéine recombinante Ag0910
• Nom complet: distal-less homeobox 5
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC006226
• Symbole du gène: DLX5
• Identification du gène (NCBI): 1749
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DLX5 belongs to the DLX family of homeobox transcription factors (Dlx1, Dlx2, Dlx5 and Dlx6), which is expressed in overlapping domains at different stages of cell differentiation in the subpallium and controls differentiation of GABAergic neurons. DLX5 is an immediate early BMP-responsive transcriptional activator that essential for osteoblast differentiation. It can bind to and stimulate the homeodomain-response element of the ALPL and SP7 promoter. DLX5 can form homodimer with DLX2(~34kd) and heterodimer with MSX2(~29kd).

Publications

Species	Application	Title
human	IHC,WB	Nucleic Acids Res; Activation of bivalent factor DLX5 cooperates with master regulator TP63 to promote squamous cell carcinoma.; Authors - Yongsheng Huang ; KD Validated
rat	WB	Acta Biomater; Osteoinductivity and biomechanical assessment of a 3D printed demineralized bone matrix-ceramic composite in a rat spine fusion model.; Authors - Mark A Plantz
mouse	WB	J Cell Physiol; Long noncoding RNA FBXL19-AS1 induces tumor growth and metastasis by sponging miR-203a-3p in lung adenocarcinoma.; Authors - Liming Wang
human	IHC	Arthritis Res Ther; Ossification process involving the human thoracic ligamentum flavum: role of transcription factors.; Authors - Uchida Kenzo K
mouse	WB	PLoS One; Involvement of microRNAs in regulation of osteoblastic differentiation in mouse induced pluripotent stem cells.; Authors - Okamoto Hideharu H