Anticorps Polyclonal de lapin anti-DLG5

DLG5 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 15687-1-AP

Synonymes

Discs large protein P dlg, Disks large homolog 5, DLG5, KIAA0583, LP DLG, P DLG5, PDLG, Placenta and prostate DLG

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Galerie de données de validation

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  WB analysis using 15687-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15687-1-AP (DLG5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 15687-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15687-1-AP (DLG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15687-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15687-1-AP (DLG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules HeLa, tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

15687-1-AP cible DLG5 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DLG5 Protéine recombinante Ag8194
• Nom complet: discs, large homolog 5 (Drosophila)
• Masse moléculaire calculée: 1893 aa, 209 kDa
• Poids moléculaire observé: 75 kDa, 240 kDa
• Numéro d’acquisition GenBank: BC002915
• Symbole du gène: DLG5
• Identification du gène (NCBI): 9231
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Discs large homolog 5 (DLG5) belongs to the membrane-associated guanylate kinase (MAGUK) family. DLG5 participates in epithelial cell polarity maintenance and cancer development. DLG5 is highly expressed in normal tissues, but its expression is decreased in many cancers, such as bladder cancer, prostate cancer, breast cancer, and hepatocellular carcinoma (PMID: 32015336). Down-regulation of DLG5 is highly correlated with clinical tumor stage. In breast cancer, knockdown of DLG5 induces cell migration, and overexpression of DLG5 inhibits cell migration (PMID: 28169360). DLG5 has 3 isoforms with the molecular weight of 75, 214 and 240 kDa.

Publications

Species	Application	Title
human	WB	Cell Death Dis; PABPC1-induced stabilization of BDNF-AS inhibits malignant progression of glioblastoma cells through STAU1-mediated decay.; Authors - Rui Su

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Rebecca (Verified Customer) (10-25-2022)

20ug of protein were loaded. Transference was performed at 4ºC and 90V for 2h. Antibody incubation was done ON at 4ºC. I obtained several bands: around 105 kDa, 200 kDa and 240 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: iPSC-CM

DLG5 Antibody Western Blot validation (1:1000 dilution) in iPSC-CM (Cat no:15687-1-AP)