Anticorps Polyclonal de lapin anti-DLD

• Phare
• Validé par KD/KO

DLD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

21

Avis (1)

N° de cat : 16431-1-AP

Synonymes

PHE3, LAD, GCSL, EC:1.8.1.4, DLDH

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Galerie de données de validation

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  WB analysis of HeLa using 16431-1-AP

  WB result of DLD antibody (16431-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DLD transfected HeLa cells.

• 

  WB analysis using 16431-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16431-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16431-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16431-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16431-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 16431-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16431-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16431-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16431-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 16431-1-AP

  IP result of anti-DLD (IP:16431-1-AP, 4ug; Detection:16431-1-AP 1:1000) with mouse liver tissue lysate 6000ug.

• 

  IHC staining of human liver cancer using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16431-1-AP (DLD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16431-1-AP (DLD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16431-1-AP (DLD antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16431-1-AP (DLD antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16431-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 16431-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLD antibody (16431-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules HeLa, cellules HepG2, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral de rat, tissu cérébral humain, tissu hépatique de souris, tissu hépatique humain
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cardiaque humain, tissu hépatique humain, tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 20 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

16431-1-AP cible DLD dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DLD Protéine recombinante Ag9729
• Nom complet: dihydrolipoamide dehydrogenase
• Masse moléculaire calculée: 509 aa, 54 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC018696
• Symbole du gène: DLD
• Identification du gène (NCBI): 1738
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DLD(Dihydrolipoyl dehydrogenase, mitochondrial) is also named as GCSL, LAD, PHE3 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It catalyzes the oxidation of dihydrolipoamide, hE3 uses two molecules : non-covalently bound FAD and a transiently bound substrate, NAD+. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.

Publications

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao
		Science; Copper induces cell death by targeting lipoylated TCA cycle proteins.; Authors - Peter Tsvetkov
mouse	WB	Nat Cell Biol; RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis; Authors - ; KO Validated
human	WB	J Hepatol; OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.; Authors - Weiqi Dai
human	WB	Nat Commun; Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.; Authors - Jiliang Xia ; KD Validated
human	WB	Redox Biol; Transketolase in human Müller cells is critical to resist light stress through the pentose phosphate and NRF2 pathways.; Authors - Yingying Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (07-12-2023)

Great antibody for WB. Very strong signal and very clean background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: Mouse liver

DLD Antibody Western Blot validation (1:4000 dilution) in Mouse liver (Cat no:16431-1-AP)