Anticorps Polyclonal de lapin anti-DLAT

• Phare
• Validé par KD/KO

DLAT Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

38

N° de cat : 13426-1-AP

Synonymes

DLAT, DLTA, PBC, PDC E2, PDCE2

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Galerie de données de validation

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  WB analysis using 13426-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13426-1-AP (DLAT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13426-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13426-1-AP (DLAT antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13426-1-AP

  WB result of DLAT antibody (13426-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DLAT transfected HeLa cells.

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  IP experiment of HepG2 using 13426-1-AP

  IP result of anti-DLAT (IP:13426-1-AP, 3ug; Detection:13426-1-AP 1:1000) with HepG2 cells lysate 2000ug.

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  IHC staining of human liver cancer using 13426-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13426-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 13426-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 13426-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 13426-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLAT antibody (13426-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules LNCaP, cellules MCF-7
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 27 publications below
• IHC: See 12 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

13426-1-AP cible DLAT dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, Lapin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DLAT Protéine recombinante Ag4226
• Nom complet: dihydrolipoamide S-acetyltransferase
• Masse moléculaire calculée: 647 aa, 70 kDa
• Poids moléculaire observé: 69-70 kDa
• Numéro d’acquisition GenBank: BC039084
• Symbole du gène: DLAT
• Identification du gène (NCBI): 1737
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao
mouse	WB	Nat Cell Biol; RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis; Authors -
human,mouse	WB,IF,CoIP	Mol Cell; Acetyl-CoA production by Mediator-bound 2-ketoacid dehydrogenases boosts de novo histone acetylation and is regulated by nitric oxide; Authors - Marta Russo
human	IHC	Cell Biosci; A new thinking: deciphering the aberrance and clinical implication of copper-death signatures in clear cell renal cell carcinoma; Authors - Aimin Jiang
human	WB,IHC	Cancer Cell Int; Molecular subtypes based on cuproptosis-related genes and tumor microenvironment infiltration characteristics in pancreatic adenocarcinoma; Authors - Jian Li
		J Biol Chem; Decreased pyruvate dehydrogenase activity in Tafazzin-deficient cells is caused by dysregulation of pyruvate dehydrogenase phosphatase 1 (PDP1); Authors - Zhuqing Liang