Anticorps Polyclonal de lapin anti-DIRAS2

DIRAS2 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 15557-1-AP

Synonymes

Di Ras2, DIRAS2, GTP binding protein Di Ras2

---

Galerie de données de validation

• 

  WB analysis of human brain using 15557-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15557-1-AP (DIRAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15557-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15557-1-AP (DIRAS2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 15557-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15557-1-AP (DIRAS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15557-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15557-1-AP (DIRAS2 antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 15557-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15557-1-AP (DIRAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15557-1-AP

  IP result of anti-DIRAS2 (IP:15557-1-AP, 4ug; Detection:15557-1-AP 1:1000) with mouse brain tissue lysate 4400ug.

• 

  IF Staining of HeLa using 15557-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15557-1-AP (DIRAS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 15557-1-AP

  Immunofluorescent analysis of Hela cells, using DIRAS2 antibody 15557-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

15557-1-AP cible DIRAS2 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DIRAS2 Protéine recombinante Ag7926
• Nom complet: DIRAS family, GTP-binding RAS-like 2
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC008065
• Symbole du gène: DIRAS2
• Identification du gène (NCBI): 54769
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cancers (Basel); Frequent Epigenetic Inactivation of DIRAS-1 and DIRAS-2 Contributes to Chemo-Resistance in Gliomas.; Authors - Tanja Rothhammer-Hampl
human,mouse	WB,IF	J Neural Transm (Vienna); Expression of the ADHD candidate gene Diras2 in the brain.; Authors - Lena Grünewald
human	WB	Dev Cell; The UBE2F-CRL5ASB11-DIRAS2 axis is an oncogene and tumor suppressor cascade in pancreatic cancer cells; Authors - Yu Chang