Anticorps Polyclonal de lapin anti-DIRAS1

• Phare
• Validé par KD/KO

DIRAS1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 12634-1-AP

Synonymes

Di Ras1, DIRAS1, GBTS1, GTP binding protein Di Ras1, RIG

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Galerie de données de validation

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  WB analysis of human brain using 12634-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12634-1-AP (DIRAS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 12634-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12634-1-AP (DIRAS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12634-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12634-1-AP (DIRAS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12634-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12634-1-AP (DIRAS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12634-1-AP

  IP result of anti-DIRAS1 (IP:12634-1-AP, 4ug; Detection:12634-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

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  IHC staining of human oesophagus using 12634-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 12634-1-AP (DIRAS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus using 12634-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 12634-1-AP (DIRAS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules MCF-7, tissu placentaire humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu œsophagien humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below

Informations sur le produit

12634-1-AP cible DIRAS1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DIRAS1 Protéine recombinante Ag3329
• Nom complet: DIRAS family, GTP-binding RAS-like 1
• Masse moléculaire calculée: 198 aa, 22 kDa
• Poids moléculaire observé: 22-24 kDa
• Numéro d’acquisition GenBank: BC030660
• Symbole du gène: DIRAS1
• Identification du gène (NCBI): 148252
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DIRAS1 belongs to the small guanosine triphosphatase (GTPase) Ras superfamily. DIRAS1 is frequently downregulated in human cancers due to DNA copy number loss, loss of heterozygosity (LOH), and hypermethylation of the promoter. The restoration of DIRAS1 has been reported to suppress tumor growth and metastasis in human neural tumors, esophageal cancer, colorectal cancer, and ovarian cancer.

Publications

Species	Application	Title
human	WB	Cancers (Basel); Frequent Epigenetic Inactivation of DIRAS-1 and DIRAS-2 Contributes to Chemo-Resistance in Gliomas.; Authors - Tanja Rothhammer-Hampl
human	WB,IHC	Mol Ther Nucleic Acids; RNAa and Vector-Mediated Overexpression of DIRAS1 Suppresses Tumor Growth and Migration in Renal Cell Carcinoma.; Authors - Xin Xu ; KD Validated
human	WB	J Biol Chem; The Tumor Suppressive Small GTPase DiRas1 Binds the Non-canonical Guanine Nucleotide Exchange Factor SmgGDS and Antagonizes SmgGDS Interactions with Oncogenic Small GTPases.; Authors - Carmen Bergom
rat	WB,IHC	Int J Environ Res Public Health; Effects of In Utero Exposure to Di-n-Butyl Phthalate on Testicular Development in Rat.; Authors - Tan Ma
human	WB,IHC	Cancer Biol Ther; M6A modification regulates tumor suppressor DIRAS1 expression in cervical cancer cells; Authors - Yu-Yan Wang