Anticorps Polyclonal de lapin anti-mDia1

• Phare
• Validé par KD/KO

mDia1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

17

Avis (1)

N° de cat : 20624-1-AP

Synonymes

DIAPH1, mDia1, Diaphanous related formin 1, Diaphanous-related formin-1, DIAP1, DIAPH 1

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Galerie de données de validation

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  WB analysis using 20624-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 20624-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 20624-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 20624-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 20624-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20624-1-AP

  IP result of anti-mDia1 (IP:20624-1-AP, 4ug; Detection:20624-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of mouse kidney using 20624-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20624-1-AP (mDia1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 20624-1-AP

  Immunofluorescent analysis of HepG2 cells, using DIAPH1 antibody 20624-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 20624-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using mDia1 antibody (20624-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 20624-1-AP

  Immunofluorescent analysis of Hela cells, using DIAPH1 antibody 20624-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 20624-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human mDia1 (20624-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules COS-7, cellules HUVEC, cellules MDA-MB-231, cellules NIH/3T3, tissu cardiaque humain, tissu de muscle squelettique humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu rénal de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2
• Résultats positifs en FC (Intra): cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 15 publications below
• IHC: See 3 publications below
• IF: See 8 publications below
• ChIP: See 1 publications below

Informations sur le produit

20624-1-AP cible mDia1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: mDia1 Protéine recombinante Ag14523
• Nom complet: diaphanous homolog 1 (Drosophila)
• Masse moléculaire calculée: 1272 aa, 141 kDa
• Poids moléculaire observé: 140-150 kDa, 70 kDa
• Numéro d’acquisition GenBank: BC007411
• Symbole du gène: mDia1
• Identification du gène (NCBI): 1729
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

mDia1, also known as DIAPH1 or Diap1, is a mammalian diaphanous-related formin which is implicated in multiple physical and pathological events including cytoskeletal dynamics, autosomal hearing loss, and myelodysplasia. Depending upon the cell type and position in the cell cycle, mDia1 has been shown to localize to the cell cortex, trafficking endosomes, cleavage furrow, mid-bodies, and centrosomes, the cytoplasmic microtubule-organizing center crucial for cell division. Mutation of mDia1 has been linked to microcephaly. This antibody recognizes the endogenous mDia1 mainly around 140-150 kDa, while sometimes an additional 70 kDa can also be observed which is proposed to be a fragment of 140-150 kDa molecules (26011179).

Publications

Species	Application	Title
mouse	WB,IHC,IF	Circ Res; Formin mDia1 mediates vascular remodeling via integration of oxidative and signal transduction pathways.; Authors - Touré Fatouma F
mouse	WB	Dev Cell; Force-dependent activation of actin elongation factor mDia1 protects the cytoskeleton from mechanical damage and promotes stress fiber repair.; Authors - Fernando R Valencia ; KD Validated
human	WB,IHC	J Cell Biol; Dia1 coordinates differentiation and cell sorting in a stratified epithelium.; Authors - Robert M Harmon
human	WB, IF	Mol Biol Cell; Small molecule agonists of mammalian Diaphanous-related (mDia) formins reveal an effective glioblastoma anti-invasion strategy.; Authors - Jessica D Arden
human	WB	Cells; The Cytoskeleton Effectors Rho-Kinase (ROCK) and Mammalian Diaphanous-Related (mDia) Formin Have Dynamic Roles in Tumor Microtube Formation in Invasive Glioblastoma Cells.; Authors - Kathryn N Becker
human	WB	PLoS One; A Novel Role for p115RhoGEF in Regulation of Epithelial Plasticity.; Authors - Swapnil S Kher ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sammy (Verified Customer) (11-20-2023)

Detects a band around 150kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000
• Cell Tissue Type: HUVEC

mDia1 Antibody Western Blot validation (1 in 1000 dilution) in HUVEC (Cat no:20624-1-AP)