Anticorps Polyclonal de lapin anti-DHX38

• Phare
• Validé par KD/KO

DHX38 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 10098-2-AP

Synonymes

DDX38, DEAH box protein 38, DHX38, KIAA0224, PRP16, PRPF16

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Galerie de données de validation

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  WB analysis using 10098-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10098-2-AP (DHX38 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10098-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10098-2-AP (DHX38 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10098-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10098-2-AP (DHX38 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10098-2-AP

  IP result of anti-DHX38 (IP:10098-2-AP, 4ug; Detection:10098-2-AP 1:700) with HeLa cells lysate 1400ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

10098-2-AP cible DHX38 dans les applications de WB, IP, IF, IHC, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DHX38 Protéine recombinante Ag0144
• Nom complet: DEAH (Asp-Glu-Ala-His) box polypeptide 38
• Masse moléculaire calculée: 141 kDa
• Poids moléculaire observé: 141 kDa
• Numéro d’acquisition GenBank: BC004235
• Symbole du gène: DHX38
• Identification du gène (NCBI): 9785
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The protein encoded by this gene is a member of the DEAD/H box family of splicing factors, which are involved in pre-mRNA splicing. The yeast prp16 is an essential splicing factor that catalyzes RNA-dependent hydrolysis of nucleoside triphosphates, acting as an ATPase. This protein resembles yeast Prp16 more closely than other DEAD/H family members and also is a general splicing factor essential for catalytic step II during pre-mRNA processing. Western blot analysis showed it is a 140-KD nuclear protein expressed in various tissues.

Publications

Species	Application	Title
human	WB	Gastroenterology; Solute Carrier Organic Anion Transporter Family Member 3A1 Is a Bile Acid Efflux Transporter in Cholestasis.; Authors - Qiong Pan
mouse	WB,IF,IP,ChIP	Nat Cell Biol; Blimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cells.; Authors - Ancelin Katia K
		Stem Cells; Characterisation of the Epigenetic Changes During Human Gonadal Primordial Germ Cells Reprogramming.; Authors - C Eguizabal
human	WB	iScience; The splicing factor DHX38 enables retinal development through safeguarding genome integrity; Authors - Kui Sun ; KD Validated
mouse	IF	PLoS One; Reprogramming primordial germ cells into pluripotent stem cells.; Authors - Durcova-Hills Gabriela G
human	WB	FEBS Open Bio; The splicing factor DHX38/PRP16 is required for ovarian clear cell carcinoma tumorigenesis, as revealed by a CRISPR-Cas9 screen.; Authors - Brandon Cona ; KD Validated