Anticorps Polyclonal de lapin anti-DHX36

• Phare
• Validé par KD/KO

DHX36 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IF, RIP, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 13159-1-AP

Synonymes

DDX36, DEAH box protein 36, DHX36, G4R1, KIAA1488, MLE like protein 1, MLEL1, RHAU

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Galerie de données de validation

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  WB analysis using 13159-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13159-1-AP (DHX36 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 13159-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13159-1-AP (DHX36 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13159-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13159-1-AP (DHX36 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 13159-1-AP

  IP result of anti-DHX36 (IP:13159-1-AP, 4ug; Detection:13159-1-AP 1:500) with A431 cells lysate 1000 ug.

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  IP experiment of PC-3 using 13159-1-AP

  IP result of anti-DHX36 (IP:13159-1-AP, 4ug; Detection:13159-1-AP 1:500) with PC-3 cells lysate 1760 ug.

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  IHC staining of human prostate cancer using 13159-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13159-1-AP (DHX36 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 13159-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13159-1-AP (DHX36 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13159-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13159-1-AP (DHX36 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13159-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13159-1-AP (DHX36 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules HEK-293T, cellules PC-3, tissu cérébral humain
• Résultats positifs en IP: cellules PC-3, cellules A431
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 2 publications below

Informations sur le produit

13159-1-AP cible DHX36 dans les applications de WB, IP, IF, RIP, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DHX36 Protéine recombinante Ag3799
• Nom complet: DEAH (Asp-Glu-Ala-His) box polypeptide 36
• Masse moléculaire calculée: 1008 aa, 115 kDa
• Poids moléculaire observé: 110-120 kDa
• Numéro d’acquisition GenBank: BC036035
• Symbole du gène: DHX36
• Identification du gène (NCBI): 170506
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DHX36, also known as RNA associated with AU-rich element (RHAU) and G4R1, is an adenosine triphosphate (ATP)-dependent RNA helicase that belongs to the DExH/D family of RNA modifying enzymes. Proteins in this family involve in a wide range of cellular functions including RNA splicing, ribosome assembly, messenger RNA (mRNA) stability, microRNA processing, ribonucleoprotein remodeling and RNA trafficking. DHX36 is an RNA helicase that has specificity for DNA and RNA G4-quadruplexes, and resolves G4 structures into separate nucleotide strands for metabolic processing of G-rich sequence.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Pseudouridine-modified tRNA fragments repress aberrant protein synthesis and predict leukaemic progression in myelodysplastic syndrome.; Authors - Nicola Guzzi
human	WB	Nat Commun; Identification of functional tetramolecular RNA G-quadruplexes derived from transfer RNAs.; Authors - Shawn M Lyons
mouse	IF	Nat Commun; Translational control by DHX36 binding to 5'UTR G-quadruplex is essential for muscle stem-cell regenerative functions.; Authors - Xiaona Chen ; KO Validated
human	WB	Nucleic Acids Res; A 5' UTR GGN repeat controls localisation and translation of a potassium leak channel mRNA through G-quadruplex formation.; Authors - Connor J Maltby
human, mouse	WB	Cell Rep; Post-transcriptional Regulation of Nkx2-5 by RHAU in Heart Development.; Authors - Junwei Nie
mouse	WB,RIP	EMBO Rep; SMaRT lncRNA controls translation of a G-quadruplex-containing mRNA antagonizing the DHX36 helicase.; Authors - Julie Martone