Anticorps Polyclonal de lapin anti-DHX15

• Phare
• Validé par KD/KO

DHX15 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 12265-1-AP

Synonymes

ATP dependent RNA helicase #46, DBP1, DDX15, DEAH box protein 15, DHX15, HRH2, PRP43, PRPF43, PrPp43p

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Galerie de données de validation

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  WB analysis using 12265-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12265-1-AP (DHX15 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12265-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12265-1-AP (DHX15 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12265-1-AP

  WB result of DHX15 antibody (12265-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX15 transfected HeLa cells.

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  WB analysis of NIH/3T3 using 12265-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12265-1-AP (DHX15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 12265-1-AP

  IP result of anti-DHX15 (IP:12265-1-AP, 4ug; Detection:12265-1-AP 1:600) with NIH/3T3 cells lysate 2000ug.

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  IHC staining of mouse testis using 12265-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12265-1-AP (DHX15 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 12265-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12265-1-AP (DHX15 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules NIH/3T3
• Résultats positifs en IP: cellules NIH/3T3
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

12265-1-AP cible DHX15 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DHX15 Protéine recombinante Ag2895
• Nom complet: DEAH (Asp-Glu-Ala-His) box polypeptide 15
• Masse moléculaire calculée: 795 aa, 91 kDa
• Poids moléculaire observé: 91 kDa
• Numéro d’acquisition GenBank: BC035974
• Symbole du gène: DHX15
• Identification du gène (NCBI): 1665
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DHX15, also named as DBP1 and DDX15, is a DExD/H-box protein that has been identified in spliceosome, through its carboxyl (C)-terminal G-patch domain and stimulates the helicase activity of DHX15. DHX15 is a Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. The MW of DHX15 is about 90-95kd.

Publications

Species	Application	Title
human,mouse	WB,IHC	Nat Commun; Fusobacterium nucleatum promotes tumor progression in KRAS p.G12D-mutant colorectal cancer by binding to DHX15; Authors - Huiyuan Zhu
zebrafish	WB	Cancer Sci; Dhx15 regulates zebrafish definitive hematopoiesis through the unfolded protein response pathway.; Authors - Yuanhua Cai
human	WB	J Cell Mol Med; ETS1 and SP1 drive DHX15 expression in acute lymphoblastic leukaemia.; Authors - Xiang-Lei Chen
human	WB	FEBS Lett; Tumor suppressor RBM5 directly interacts with the DExD/H-box protein DHX15 and stimulates its helicase activity.; Authors - Niu Zhaoyang Z
human	WB	RNA; A model for DHX15 mediated disassembly of A-complex spliceosomes; Authors - Hannah M Maul-Newby
		PLoS Pathog; Downregulation of CPSF6 Leads to Global mRNA 3' UTR shortening and enhanced antiviral immune responses; Authors - Yong Ge