Anticorps Polyclonal de lapin anti-DHODH

• Phare
• Validé par KD/KO

DHODH Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF-P, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

62

Avis (2)

N° de cat : 14877-1-AP

Synonymes

PYRD, EC:1.3.5.2, Dihydroorotate oxidase, Dihydroorotate dehydrogenase (quinone), mitochondrial, dihydroorotate dehydrogenase

---

Galerie de données de validation

• 

  WB analysis using 14877-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14877-1-AP (DHODH antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 14877-1-AP

  IP result of anti-DHODH (IP:14877-1-AP, 4ug; Detection:14877-1-AP 1:1000) with mouse spleen tissue lysate 4000ug.

• 

  IHC staining of human breast cancer using 14877-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14877-1-AP (DHODH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14877-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14877-1-AP (DHODH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 14877-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using DHODH antibody (14877-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293T using 14877-1-AP

  1X10^6 HEK-293T cells were intracellularly stained with 0.2 ug Anti-Human DHODH (14877-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules A2780, cellules MCF-7, cellules SKOV-3, tissu cardiaque de souris, tissu ovarien de souris, tissu splénique de rat, tissu splénique de souris
• Résultats positifs en IP: tissu splénique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu rénal de souris,
• Résultats positifs en FC (Intra): cellules HEK-293T,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 13 publications below
• WB: See 57 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

14877-1-AP cible DHODH dans les applications de WB, IHC, IF-P, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DHODH Protéine recombinante Ag6649
• Nom complet: dihydroorotate dehydrogenase
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC065245
• Symbole du gène: DHODH
• Identification du gène (NCBI): 1723
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DHODH(Dihydroorotate dehydrogenase) catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis.DHO dehydrogenase is a monofunctional protein which, in most eukaryotic organisms, is located on the outer surface of the inner mitochondrial membrane.Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS).

Publications

Species	Application	Title
human	WB	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
human	WB	Nature; DHODH-mediated ferroptosis defence is a targetable vulnerability in cancer.; Authors - Chao Mao ; KO Validated
human,mouse	WB	Science; Stimulation of de novo pyrimidine synthesis by growth signaling through mTOR and S6K1.; Authors - Issam Ben-Sahra
human,mouse	WB	Cancer Discov; PTEN Regulates Glutamine Flux to Pyrimidine Synthesis and Sensitivity to Dihydroorotate Dehydrogenase Inhibition.; Authors - Deepti Mathur
mouse	IHC	Cancer Cell; Cancer-selective metabolic vulnerabilities in MYC-amplified medulloblastoma; Authors - William D Gwynne ; KO Validated
mouse	WB	Cell Metab; Macrophage-Released Pyrimidines Inhibit Gemcitabine Therapy in Pancreatic Cancer.; Authors - Christopher J Halbrook ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Kristian (Verified Customer) (07-06-2021)

Paraffin embedded kidneys were stained for Dhodh (green) and DAPI (blue)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse kidney

DHODH Antibody Immunofluorescence validation (1:200 dilution) in Mouse kidney (Cat no:14877-1-AP)

FH

Daniel (Verified Customer) (04-16-2019)

Highly specific detection of both mouse and human DHODH.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human RCC4 cells (lane1), Mouse kidney (lane2)

DHODH Antibody Western Blot, validation (1:1000 dilution) in Human RCC4 cells (lane1), Mouse kidney (lane2) (Cat no:14877-1-AP)