Anticorps Polyclonal de lapin anti-DFNA5/GSDME

• Phare
• Validé par KD/KO

DFNA5/GSDME Polyclonal Antibody for WB, IP, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IF, IHC, CoIP, ELISA, FC (Intra)

Conjugaison

Non conjugué

Publications

34

Avis (2)

N° de cat : 13075-1-AP

Synonymes

Cleaved Gasdermin E, deafness, autosomal dominant 5, DFNA5, DFNA5/GSDME, Gasdermin E, Gasdermin-E, GSDME, ICERE 1, ICERE1

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Galerie de données de validation

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  WB analysis using 13075-1-AP

  Untreated, and etoposide (60uM, 5h) treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13075-1-AP (DFNA5/GSDME antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13075-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13075-1-AP (DFNA5/GSDME antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 13075-1-AP

  WB result of DFNA5/ GSDME antibody (13075-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-DFNA5/ GSDME transfected A549 cells.

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  WB analysis using 13075-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13075-1-AP (DFNA5/GSDME antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 13075-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13075-1-AP (DFNA5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 13075-1-AP

  IP result of anti-DFNA5/GSDME (IP:13075-1-AP, 4ug; Detection:13075-1-AP 1:15000) with SH-SY5Y cells lysate 1240 ug.

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  IHC staining of mouse brain using 13075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13075-1-AP (DFNA5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13075-1-AP (DFNA5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 13075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13075-1-AP (DFNA5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 13075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13075-1-AP (DFNA5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of SH-SY5Y using 13075-1-AP

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human DFNA5/GSDME (13075-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules A549, cellules HeLa, cellules Y79
• Résultats positifs en IP: cellules SH-SY5Y,
• Résultats positifs en IHC: tissu cérébral de souris, tissu d'intestin grêle de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules SH-SY5Y
• Résultats positifs en cytométrie: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 22 publications below
• IHC: See 14 publications below
• IF: See 7 publications below
• CoIP: See 1 publications below

Informations sur le produit

13075-1-AP cible DFNA5/GSDME dans les applications de WB, IP, IF, IHC, CoIP, ELISA, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DFNA5/GSDME Protéine recombinante Ag3746
• Nom complet: deafness, autosomal dominant 5
• Masse moléculaire calculée: 496 aa, 55 kDa
• Poids moléculaire observé: 55 kDa, 35 kDa, 25 kDa
• Numéro d’acquisition GenBank: BC019689
• Symbole du gène: DFNA5
• Identification du gène (NCBI): 1687
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain. GSDME produced two GSDME fragments with MW of 35 kDa and 25 kDa.

Publications

Species	Application	Title
human,mouse	WB,IHC,CoIP	Cell Res; Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P; Authors - Yuan-Li Ai
human	IF	Acta Pharm Sin B; A high-throughput Gaussia luciferase reporter assay for screening potential gasdermin E activators against pancreatic cancer; Authors - Yang Liu
mouse	WB	J Control Release; Enhancing cell pyroptosis with biomimetic nanoparticles for melanoma chemo-immunotherapy; Authors - Shiquan Sun
mouse	IF	Cell Death Differ; GSDME-mediated pyroptosis promotes inflammation and fibrosis in obstructive nephropathy.; Authors - Yinshuang Li
mouse	WB,IHC	J Nanobiotechnology; Hydralazine loaded nanodroplets combined with ultrasound-targeted microbubble destruction to induce pyroptosis for tumor treatment; Authors - Shuting Huang
		Cell Death Dis; TNFSF15 inhibits progression of diabetic retinopathy by blocking pyroptosis via interacting with GSDME; Authors - Hongkun Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

James (Verified Customer) (11-18-2022)

Used for GSDME detection in splenocyte extracts

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Spleen

DFNA5/GSDME Antibody Western Blot validation (1/1000 dilution) in Spleen (Cat no:13075-1-AP)

FH

Evelina (Verified Customer) (03-14-2022)

Good antibody to detect GSDME in cell lysates. Although does detect one of the proteins within the ladder (approx.15kDa) when using stronger ECL.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

DFNA5/GSDME Antibody Western Blot validation (1:1000 dilution) in (Cat no:13075-1-AP)

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C