Anticorps Polyclonal de lapin anti-DEPDC6/Deptor

DEPDC6/Deptor Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 20985-1-AP

Synonymes

DEP domain containing 6, DEP.6, DEPDC6, DEPDC6/Deptor, Deptor

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Galerie de données de validation

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  WB analysis of HeLa using 20985-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 20985-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20985-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 20985-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 20985-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20985-1-AP (DEPDC6/Deptor antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20985-1-AP (DEPDC6/Deptor antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human thyroid cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human thyroid cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 20985-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DEPDC6/Deptor antibody (20985-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A2780, cellules COLO 320, cellules HEK-293, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer de la thyroïde humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

20985-1-AP cible DEPDC6/Deptor dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: DEP domain containing 6
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: NM_022783
• Symbole du gène: DEPDC6
• Identification du gène (NCBI): 64798
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DEPDC6, also named as DEPTOR, was identified as a novel mTOR-interacting protein whose over-expression in certain cell types represents a new mechanism for activating PI3K/Akt signaling and promoting cell survival. It negative regulator of the mTORC1 and mTORC2 signaling pathways. DEPDC6 inhibits the kinase activity of both complexes. The antibody is specific to DEPDC6.

Publications

Species	Application	Title
human	WB,IHC	Sci Transl Med; The PTH/PTHrP-SIK3 pathway affects skeletogenesis through altered mTOR signaling.; Authors - Fabiana Csukasi
human	WB	Autophagy; TNFAIP3-DEPTOR complex regulates inflammasome secretion through autophagy in ankylosing spondylitis monocytes.; Authors - Yue Zhai
human	WB	Cell Death Differ; NUMB facilitates autophagy initiation through targeting SCFβ-TrCP2 complex.; Authors - Hao Li
mouse	WB	Oncotarget; Inhibition of neddylation regulates dendritic cell functions via Deptor accumulation driven mTOR inactivation.; Authors - Mengmeng Cheng
mouse	IF	Stem Cell Res Ther; DEPTOR regulates osteogenic differentiation via inhibiting MEG3-mediated activation of BMP4 signaling and is involved in osteoporosis.; Authors - Si Chen
mouse	WB	Gene; p65 down-regulates DEPTOR expression in response to LPS stimulation in hepatocytes.; Authors - Xiaoling Yu