Anticorps Polyclonal de lapin anti-DENR

• Phare
• Validé par KD/KO

DENR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 10656-1-AP

Synonymes

SMAP 3, Protein DRP1, H14, DRP1, DRP

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Galerie de données de validation

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  WB analysis of A431 using 10656-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10656-1-AP (DENR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10656-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10656-1-AP (DENR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 10656-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10656-1-AP (DENR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10656-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10656-1-AP (DENR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10656-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10656-1-AP (DENR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 10656-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10656-1-AP (DENR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10656-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10656-1-AP (DENR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10656-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10656-1-AP (DENR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 10656-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DENR antibody (10656-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 10656-1-AP

  Immunofluorescent analysis of A431 cells using 10656-1-AP (DENR antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, tissu cérébral de souris, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293, cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 12 publications below

Informations sur le produit

10656-1-AP cible DENR dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, poisson-zèbre, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DENR Protéine recombinante Ag1023
• Nom complet: density-regulated protein
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 27-30 kDa
• Numéro d’acquisition GenBank: BC007860
• Symbole du gène: DENR
• Identification du gène (NCBI): 8562
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DENR, also named as SMAP-3 or DRP1, is a 198 amino acid protein, which is highly expressed in heart and skeletal muscle and moderately expressed in the brain, placenta, liver and pancreas. DENR is Up-regulated with increasing cell-density by HNRNPD and Up-regulated in ovarian and breast cancer cells by ERBB2 overexpression. The calculated molecular weight of DENR is 22 kDa, but the modified protein is about 25-30 kDa. (PMID: 27239039 )

Publications

Species	Application	Title
human,mouse	WB	Nat Commun; DENR controls JAK2 translation to induce PD-L1 expression for tumor immune evasion.; Authors - Baiwen Chen
human	WB	Cell Rep; CRISPR screening reveals a dependency on ribosome recycling for efficient SARS-CoV-2 programmed ribosomal frameshifting and viral replication; Authors - Frederick Rehfeld ; KD Validated
human	WB	Sci Rep; A screening strategy for the discovery of drugs that reduce C/EBPβ-LIP translation with potential calorie restriction mimetic properties.; Authors - Mohamad A Zaini ; KO Validated
zebrafish	WB	Nutrients; A High-Fat Diet Induces Muscle Mitochondrial Dysfunction and Impairs Swimming Capacity in Zebrafish: A New Model of Sarcopenic Obesity.; Authors - Yun-Yi Zou
rat	WB	J Cell Mol Med; A possible new activator of PI3K-Huayu Qutan Recipe alleviates mitochondrial apoptosis in obesity rats with acute myocardial infarction.; Authors - He Tai
rat	WB	Front Med (Lausanne); Tanshinone IIA Combined With Cyclosporine A Alleviates Lung Apoptosis Induced by Renal Ischemia-Reperfusion in Obese Rats.; Authors - He Tai