Anticorps Polyclonal de lapin anti-RIG-1/DDX58

RIG-1/DDX58 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 25068-1-AP

Synonymes

DDX58, DEAD box protein 58, RIG 1, RIG I, RIG-1/DDX58, RIG-I

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Galerie de données de validation

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  WB analysis using 25068-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25068-1-AP (RIG-1/DDX58 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 25068-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25068-1-AP (DDX58 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25068-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25068-1-AP (RIG-1/DDX58 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25068-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25068-1-AP (RIG-1/DDX58 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 25068-1-AP

  IP result of anti-RIG-1/DDX58 (IP:25068-1-AP, 4ug; Detection:25068-1-AP 1:600) with A431 cells lysate 1200ug.

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  IHC staining of human heart using 25068-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25068-1-AP (DDX58 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 25068-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25068-1-AP (DDX58 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 25068-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25068-1-AP (DDX58 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 25068-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25068-1-AP (DDX58 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules NIH/3T3, cellules THP-1
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: tissu cardiaque humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

25068-1-AP cible RIG-1/DDX58 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RIG-1/DDX58 Protéine recombinante Ag18585
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
• Masse moléculaire calculée: 925 aa, 106 kDa
• Poids moléculaire observé: 101~106 kDa
• Numéro d’acquisition GenBank: BC132786
• Symbole du gène: RIG-1/DDX58
• Identification du gène (NCBI): 23586
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.

Publications

Species	Application	Title
human	WB,IHC	Cancer Med; RIG-I promotes IFN/JAK2 expression and the endoplasmic reticulum stress response to inhibit chemoradiation resistance in nasopharyngeal carcinoma.; Authors - Di Jing
	WB	bioRxiv; A tale of two tumors: differential, but detrimental, effects of glioblastoma extracellular vesicles (EVs) on normal human brain cells; Authors - Mary Wang
human	WB,IP	bioRxiv; IFIH1 (MDA5) is required for innate immune detection of intron-containing RNA expressed from the HIV-1 provirus; Authors - Mehmet Hakan Guney