Anticorps Polyclonal de lapin anti-DDX51

DDX51 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA, IF

Conjugaison

Non conjugué

Publications

1

N° de cat : 20149-1-AP

Synonymes

EC:3.6.4.13, DEAD box protein 51, ATP-dependent RNA helicase DDX51

---

Galerie de données de validation

• 

  WB analysis using 20149-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 20149-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 20149-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 20149-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 20149-1-AP

  IP result of anti-DDX51 (IP:20149-1-AP, 4ug; Detection:20149-1-AP 1:1000) with K-562 cells lysate 1820 ug.

---

Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules K-562, cellules Raji
• Résultats positifs en IP: cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

20149-1-AP cible DDX51 dans les applications de WB, IP, ELISA, IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDX51 Protéine recombinante Ag13691
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 51
• Masse moléculaire calculée: 666 aa, 72 kDa
• Poids moléculaire observé: 72-80 kDa
• Numéro d’acquisition GenBank: BC040185
• Symbole du gène: DDX51
• Identification du gène (NCBI): 317781
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The DEAD-box family (DDX) consists of a group of RNA helicases with the activity to hydrolyze ATP and to unwind RNA. The major physiological role of this family was to regulate RNA metabolism including ribosome assembly, RNA splicing, and pre-rRNA processing. DDX51 was a new gene in DDX family composed of 666 amino acids. DDX51 gene was a member of RNA helicases family in charge of regulating RNA metabolism(PMID: 31966432).

Publications

Species	Application	Title
human	IF	Nat Commun; Intracellular energy controls dynamics of stress-induced ribonucleoprotein granules; Authors - Tao Wang