Anticorps Polyclonal de lapin anti-DDX50

• Phare
• Validé par KD/KO

DDX50 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 10358-1-AP

Synonymes

DDX50, DEAD box protein 50, Gu beta, GU2, GUB, Nucleolar protein Gu2, RH II/GuB

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Galerie de données de validation

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  WB analysis of HeLa using 10358-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10358-1-AP (DDX50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10358-1-AP

  WB result of DDX50 antibody (10358-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX50 transfected HeLa cells.

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  WB analysis of Jurkat using 10358-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10358-1-AP (DDX50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10358-1-AP

  IP result of anti-DDX50 (IP:10358-1-AP, 4ug; Detection:10358-1-AP 1:800) with HeLa cells lysate 1200ug.

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  IF Staining of HeLa using 10358-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX50 antibody (10358-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

10358-1-AP cible DDX50 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDX50 Protéine recombinante Ag0382
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 50
• Masse moléculaire calculée: 83 kDa
• Poids moléculaire observé: 83 kDa
• Numéro d’acquisition GenBank: BC000272
• Symbole du gène: DDX50
• Identification du gène (NCBI): 79009
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DEAD box polypeptide 50 (DDX50, synonyms: GU2, GUB, MGC3199, RH-II/GuB) enzyme may be involved in ribosomal RNA synthesis or processing.

Publications

Species	Application	Title
human	WB	Cell Chem Biol; Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.; Authors - Li Wang
human	WB	Arch Virol; DDX50 inhibits the replication of dengue virus 2 by upregulating IFN-β production.; Authors - Peijun Han
human	WB	Mol Cell; Nucleolus activity-dependent recruitment and biomolecular condensation by pH sensing; Authors - Fardin Aryan