Anticorps Recombinant de lapin anti-DDX3

• Phare
• Validé par KD/KO

DDX3 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

5C12

N° de cat : 81903-1-RR

Synonymes

DDX3X, 5C12, ATP-dependent RNA helicase DDX3X, CAP-Rf, DBX

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Galerie de données de validation

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  WB analysis of HepG2 using 81903-1-RR

  WB result of DDX3 antibody (81903-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX3 transfected HepG2 cells.

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  WB analysis using 81903-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81903-1-RR (DDX3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 81903-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DDX3 antibody (81903-1-RR, Clone: 5C12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 81903-1-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug DDX3 Recombinant antibody (81903-1-RR, Clone:5C12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules K-562, cellules MCF-7, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

81903-1-RR cible DDX3 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DDX3 Protéine recombinante Ag1614
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC011819
• Symbole du gène: DDX3
• Identification du gène (NCBI): 1654
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDX3X, also named as DBX, DDX3 and HLP2, belongs to the DEAD box helicase family and DDX3/DED1 subfamily. It is ATP-dependent RNA helicase. DDX3X acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It is also involved in HIV-1 replication. DDX3X interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. 73 kDa is the target band. 60-65 kDa is some other isoform. This antibody can bind both DDX3X and DDX3Y for the close sequences.

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