Anticorps Polyclonal de lapin anti-DDX27

DDX27 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 17087-1-AP

Synonymes

DDX27, DEAD box protein 27, HSPC259, PP3241, RHLP, Rrp3p

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Galerie de données de validation

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  WB analysis of HeLa using 17087-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17087-1-AP (DDX27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17087-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17087-1-AP (DDX27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 17087-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17087-1-AP (DDX27 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 17087-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17087-1-AP (DDX27 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules NIH/3T3, tissu hépatique de souris
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

17087-1-AP cible DDX27 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDX27 Protéine recombinante Ag9426
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 27
• Masse moléculaire calculée: 796 aa, 90 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC016060
• Symbole du gène: DDX27
• Identification du gène (NCBI): 55661
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDX27 is one of the DEAD box proteins, which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). DEAD box proteins are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division, and DDX27 is involved in the processing of 5.8S and 28S ribosomal RNAs.

Publications

Species	Application	Title
human	WB	Mol Cell; Circular RNA ZNF609/CKAP5 mRNA interaction regulates microtubule dynamics and tumorigenicity.; Authors - Francesca Rossi
human	WB	Onco Targets Ther; DEAD-box helicase 27 plays a tumor-promoter role by regulating the stem cell-like activity of human colorectal cancer cells.; Authors - Chunxing Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wael (Verified Customer) (05-24-2023)

we detected the relative abundance of DDX27 in both the Nuclear and cytoplasmic fractions

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hek293

DDX27 Antibody Western Blot validation (1:1000 dilution) in Hek293 (Cat no:17087-1-AP)