Anticorps Polyclonal de lapin anti-DDX17

• Phare
• Validé par KD/KO

DDX17 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA, PLA

Conjugaison

Non conjugué

Publications

15

Avis (2)

N° de cat : 19910-1-AP

Synonymes

DDX17, DEAD box protein 17, DEAD box protein p72, P72, RH70, RNA dependent helicase p72

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Galerie de données de validation

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  WB analysis using 19910-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19910-1-AP (DDX17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19910-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19910-1-AP (DDX17 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19910-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19910-1-AP (DDX17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19910-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19910-1-AP (DDX17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 19910-1-AP

  IP result of anti-DDX17 (IP:19910-1-AP, 4ug; Detection:19910-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human kidney using 19910-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19910-1-AP (DDX17 antibody) at dilution of 1:1000 (under 40x lens).

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  IHC staining of human kidney using 19910-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19910-1-AP (DDX17 antibody) at dilution of 1:800 (under 10x lens).

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  IHC staining of human kidney using 19910-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19910-1-AP (DDX17 antibody) at dilution of 1:800 (under 40x lens).

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  IHC staining of human kidney using 19910-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19910-1-AP (DDX17 antibody) at dilution of 1:1000 (under 10x lens).

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  IF Staining of HEK-293 using 19910-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DDX17 antibody (19910-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HEK-293, cellules HEK-293T, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 6 publications below
• CoIP: See 2 publications below
• ChIP: See 3 publications below

Informations sur le produit

19910-1-AP cible DDX17 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA, PLA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDX17 Protéine recombinante Ag13723
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 17
• Masse moléculaire calculée: 729 aa, 80 kDa
• Poids moléculaire observé: 72-80 kDa
• Numéro d’acquisition GenBank: BC000595
• Symbole du gène: DDX17
• Identification du gène (NCBI): 10521
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDX17, also named as DEAD box protein p72 and DEAD box protein p82, is a 729 amino acid protein, which belongs to the DEAD box helicase family. DDX5/DBP2 subfamily. DDX17 is widely expressed. Levels tend to increase during colon cancer progression, from very low in benign hyperplastic polyps to very high in tubular and villous adenomas. DDX17 as an RNA helicase unwinds RNA and alters RNA structures through ATP binding and hydrolysis. DDX17 is involved in multiple cellular processes, including pre-mRNA splicing, alternative splicing, ribosomal RNA processing and miRNA processing, as well as transcription regulation. It Regulates the alternative splicing of exons exhibiting specific features. identification and characterisation of p72, a novel human nuclear DEAD box protein, which shows a striking homology to p68. a predicted molecular mass of DDX17 is 72 kDa, but endogenous DDX17 migrates aberrantly at 79 kDa on SDS-PAGE (PMID: 8871553).

Publications

Species	Application	Title
human	CoIP,chIP	Nat Commun; Intragenic recruitment of NF-κB drives splicing modifications upon activation by the oncogene Tax of HTLV-1.; Authors - Lamya Ben Ameur
human	IP	Nucleic Acids Res; The RNA helicase DDX17 controls the transcriptional activity of REST and the expression of proneural microRNAs in neuronal differentiation.; Authors - Marie-Pierre Lambert
human	WB,IP	Nucleic Acids Res; RNA helicase-dependent gene looping impacts messenger RNA processing; Authors - Sophie Terrone ; KD Validated
mouse,human	WB,IHC	Clin Transl Med; DEAD-Box Helicase 17 exacerbates non-alcoholic steatohepatitis via transcriptional repression of cyp2c29, inducing hepatic lipid metabolism disorder and eliciting the activation of M1 macrophages; Authors - Deng Ning
human	WB	PLoS Pathog; Hepatitis B virus Core protein nuclear interactome identifies SRSF10 as a host RNA-binding protein restricting HBV RNA production.; Authors - Hélène Chabrolles
human	IF	J Biol Chem; RNA Polymerase III Regulates Cytosolic RNA:DNA Hybrids and Intracellular MicroRNA Expression.; Authors - Christine Xing'er Koo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Roy (Verified Customer) (06-15-2024)

Great antibody to detect DDX17 by WB (Overnight incubation at 4°C, 1/1000 dilution)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa

FH

N (Verified Customer) (01-28-2023)

Perfect to detect double bands of DDX17.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

DDX17 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:19910-1-AP)