Anticorps Polyclonal de lapin anti-DDX1

• Phare
• Validé par KD/KO

DDX1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, CoIP, RIP, ELISA, IF

Conjugaison

Non conjugué

Publications

20

N° de cat : 11357-1-AP

Synonymes

DBP RB, DDX1, DEAD box protein 1, UKVH5d

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Galerie de données de validation

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  WB analysis using 11357-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11357-1-AP (DDX1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11357-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11357-1-AP (DDX1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 11357-1-AP

  IP result of anti-DDX1 (IP:11357-1-AP, 3ug; Detection:11357-1-AP 1:1000) with PC-3 cells lysate 5000ug.

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  IHC staining of human lymphoma using 11357-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11357-1-AP (DDX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 11357-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11357-1-AP (DDX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11357-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11357-1-AP (DDX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 11357-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11357-1-AP (DDX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 11357-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11357-1-AP (DDX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 11357-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11357-1-AP (DDX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 11357-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 11357-1-AP (DDX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 11357-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 11357-1-AP (DDX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 11357-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 11357-1-AP (DDX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A431, cellules HeLa, cellules MCF-7, cellules NCI-H1299, cellules NIH/3T3, cellules PC-3, tissu cérébral de rat, tissu cérébral de souris, tissu rénal de rat, tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

11357-1-AP cible DDX1 dans les applications de WB, IHC, IP, CoIP, RIP, ELISA, IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDX1 Protéine recombinante Ag1887
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
• Masse moléculaire calculée: 740 aa, 82 kDa
• Poids moléculaire observé: 70-82 kDa
• Numéro d’acquisition GenBank: BC012132
• Symbole du gène: DDX1
• Identification du gène (NCBI): 1653
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDX1 is a DEAD box protein, which is putative RNA helicases with a characteristic asp-glu-ala-asp (DEAD) box motif. DEAD box proteins involve in translation initiation, splicing, and ribosome and spliceosome assembly by altering RNA secondary structure. As a RNA helicase, DDX1 has a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome.

Publications

Species	Application	Title
human	IF	Cell; Modulation of RNA Condensation by the DEAD-Box Protein eIF4A.; Authors - Devin Tauber
mouse	WB,CoIP,IP	Mol Cell; RNA Helicase DDX1 Converts RNA G-Quadruplex Structures into R-Loops to Promote IgH Class Switch Recombination.; Authors - Claudia Ribeiro de Almeida ; KD Validated
human	WB	Cell Host Microbe; RNase L Activates the NLRP3 Inflammasome during Viral Infections.; Authors - Arindam Chakrabarti ; KD Validated
mouse	WB	Nat Commun; BEX1 is an RNA-dependent mediator of cardiomyopathy.; Authors - Federica Accornero
rat	WB,IF	Nucleic Acids Res; PMID: 30295850; Authors - Zonghong Li ; KD Validated
mouse	IF	Cell Syst; BraInMap Elucidates the Macromolecular Connectivity Landscape of Mammalian Brain.; Authors - Reza Pourhaghighi