Anticorps Polyclonal de lapin anti-DDHD2

• Phare
• Validé par KD/KO

DDHD2 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 25203-1-AP

Synonymes

DDHD domain containing 2, DDHD2, KIAA0725, KIAA0725p, Phospholipase DDHD2, SAMWD1

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Galerie de données de validation

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  WB analysis of rat brain using 25203-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25203-1-AP (DDHD2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 25203-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 25203-1-AP (DDHD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 25203-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25203-1-AP (DDHD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 25203-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25203-1-AP (DDHD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25203-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25203-1-AP (DDHD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 25203-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25203-1-AP (DDHD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 25203-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25203-1-AP (DDHD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 25203-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DDHD2 antibody (25203-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HEK-293 using 25203-1-AP

  Immunofluorescent analysis of HEK-293 cells using 25203-1-AP (DDHD2 antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, tissu pulmonaire de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

25203-1-AP cible DDHD2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDHD2 Protéine recombinante Ag18373
• Nom complet: DDHD domain containing 2
• Masse moléculaire calculée: 711 aa, 81 kDa
• Poids moléculaire observé: 80-90 kDa
• Numéro d’acquisition GenBank: BC010504
• Symbole du gène: DDHD2
• Identification du gène (NCBI): 23259
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDHD2, also known as KIAA0725p, is a member of the intracellular phospholipase A1 (PLA1) protein family which comprises a group of enzymes that hydrolyze the sn-1 ester bond of phospholipids, producing 2-acyl-lysophospholipids and fatty acids. DDHD2 prefers phosphatidic acid as substrate and has a role in efficient membrane trafficking from the Golgi apparatus to the plasma membrane. The gene of DDHD2 maps to chromosome 8p11.23, and encodes a 711-amino-acid protein with a calculated molecular mass of 81 kDa. It has been reported that DDHD2 immunoblot analysis of various tissue extracts revealed two bands of 90 and 85 kDa (PMID: 11788596).

Publications

Species	Application	Title
mouse		Neuron; Rewiring Neuronal Glycerolipid Metabolism Determines the Extent of Axon Regeneration.; Authors - Chao Yang ; KD Validated
mouse	WB	J Biol Chem; The phospholipase PNPLA7 functions as a lysophosphatidylcholine hydrolase and interacts with lipid droplets through its catalytic domain.; Authors - Christoph Heier
human	WB,IHC	Cell Death Dis; Circular RNA circRUNX1 promotes papillary thyroid cancer progression and metastasis by sponging MiR-296-3p and regulating DDHD2 expression.; Authors - Junjie Chu ; KD Validated
mouse	WB,IF	J Lipid Res; Cooperative lipolytic control of neuronal triacylglycerol by spastic paraplegia-associated enzyme DDHD2 and ATGL; Authors - Peter Hofer
human	WB	Protein Cell; Proteomic analysis of ferroptosis pathways reveals a role of CEPT1 in suppressing ferroptosis; Authors - Xiaoguang Liu
rat	WB,IF,IP	EMBO J; The DDHD2-STXBP1 interaction mediates long-term memory via generation of saturated free fatty acids; Authors - Isaac O Akefe ; KO Validated