Anticorps Polyclonal de lapin anti-DOPA decarboxylase

DOPA decarboxylase Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 10166-1-AP

Synonymes

AADC, DDC, DOPA decarboxylase

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Galerie de données de validation

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  WB analysis using 10166-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10166-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10166-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10166-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10166-1-AP

  IP result of anti-DOPA decarboxylase (IP:10166-1-AP, 4ug; Detection:10166-1-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IHC staining of rat small intestine using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10166-1-AP (DOPA decarboxylase antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10166-1-AP (DOPA decarboxylase antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of SH-SY5Y using 10166-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DOPA decarboxylase antibody (10166-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules PC-12, tissu cérébral de souris, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu rénal de souris, tissu de cancer du foie humain, tissu d'intestin grêle de rat, tissu rénal de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 7 publications below
• IF: See 2 publications below

Informations sur le produit

10166-1-AP cible DOPA decarboxylase dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DOPA decarboxylase Protéine recombinante Ag0219
• Nom complet: dopa decarboxylase (aromatic L-amino acid decarboxylase)
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: BC008366
• Symbole du gène: DOPA decarboxylase
• Identification du gène (NCBI): 1644
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Aromatic-L-amino-acid decarboxylase belongs to the pyridoxal-dependent aminotransferase superfamily.DDC catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.DDC is the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD).Researches showed that Ddc is only one of the enzymes in the biosynthetic pathways for bioamines and catecholamines.

Publications

Species	Application	Title
mouse	IF	Nature; Reversing a model of Parkinson's disease with in situ converted nigral neurons.; Authors - Hao Qian
human,mouse	WB,IHC	Cell Signal; Profile of 5-HT2A receptor involved in signaling cascades associated to intracellular inflammation and apoptosis in hepatocytes and its role in carbon tetrachloride-induced hepatotoxicity; Authors - Yuxin Zhang
human	WB	Dis Model Mech; A Matrigel-based 3D construct of SH-SY5Y cells models the α-synuclein pathologies of Parkinson's disease.; Authors - Zhao-Feng Li
mouse	IHC,IF	Toxicol Appl Pharmacol; PMID: 21703292; Authors - Fox Donald A DA
human	WB	J Transl Med; Retinal pigment epithelial cells secrete neurotrophic factors and synthesize dopamine: possible contribution to therapeutic effects of RPE cell transplantation in Parkinson's disease.; Authors - Ming Ming
human,mouse	WB,IHC	Toxicol Appl Pharmacol; Carbon tetrachloride induced mitochondrial division, respiratory chain damage, abnormal intracellular [H+] and apoptosis are due to the activation of 5-HT degradation system in hepatocytes.; Authors - Yuxin Zhang