Anticorps Polyclonal de lapin anti-DDB2

DDB2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 30173-1-AP

Synonymes

DDB p48 subunit, DDB2, DDBB, DNA damage binding protein 2, FLJ34321, UV DDB 2, UV DDB2

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Galerie de données de validation

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  WB analysis using 30173-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30173-1-AP (DDB2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30173-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30173-1-AP (DDB2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30173-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30173-1-AP (DDB2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HCT 116, cellules HeLa, cellules HepG2, cellules Jurkat, cellules NIH/3T3, cellules PC-12, tissu hépatique de souris, tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

30173-1-AP cible DDB2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDB2 Protéine recombinante Ag32811
• Nom complet: damage-specific DNA binding protein 2, 48kDa
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48-51 kDa
• Numéro d’acquisition GenBank: NM_000107
• Symbole du gène: DDB2
• Identification du gène (NCBI): 1643
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDB2, also named as DNA damage-binding protein 2 or UV-damaged DNA-binding protein 2, is a 427 amino acid protein, which contains seven WD repeats and belongs to the WD repeat DDB2/WDR76 family. DDB2 is ubiquitously expressed, with highest levels in corneal endothelium and lowest levels in brain. DDB2 localizes in the nucleus and is required for DNA repair. DDB2 regulates negatively the constitutive expression of the SOD2 gene in breast cancer cells and exerts, at least in part, a control of breast cancer cell growth. DDB2 can play a role as oncogene and may become a promising candidate as a predictive marker in breast cancer.