Anticorps Polyclonal de lapin anti-DDB1

• Phare
• Validé par KD/KO

DDB1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (1)

N° de cat : 11380-1-AP

Synonymes

DDB p127 subunit, DDB1, DDBA, DNA damage binding protein 1, DNA damage binding protein a, HBV X associated protein 1, UV damaged DNA binding factor, UV DDB 1, UV DDB1, XAP 1, XAP1, XPCE, XPE, XPE BF, XPE binding factor

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Galerie de données de validation

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  WB analysis using 11380-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11380-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 11380-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 11380-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 11380-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11380-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11380-1-AP (DDB1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 11380-1-AP

  IP result of anti-DDB1 (IP:11380-1-AP, 5ug; Detection:11380-1-AP 1:500) with Jurkat cells lysate 3080ug.

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  IHC staining of human colon cancer using 11380-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11380-1-AP (DDB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11380-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11380-1-AP (DDB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11380-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11380-1-AP (DDB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11380-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11380-1-AP (DDB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules C2C12, cellules HeLa, cellules HepG2, cellules Jurkat, cellules MCF-7, cellules MDA-MB-231, cellules NIH/3T3, tissu cérébral humain, tissu placentaire humain, tissu rénal humain, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

11380-1-AP cible DDB1 dans les applications de WB, IP, IHC, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDB1 Protéine recombinante Ag1901
• Nom complet: damage-specific DNA binding protein 1, 127kDa
• Masse moléculaire calculée: 1140 aa, 127 kDa
• Poids moléculaire observé: 127 kDa
• Numéro d’acquisition GenBank: BC011686
• Symbole du gène: DDB1
• Identification du gène (NCBI): 1642
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDB1, also named as XAP1, XPCe, DDBa and XPE-BF, belongs to the DDB1 family. It is required for DNA repair. DDB1 binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. This antibody is specific to DDB1.

Publications

Species	Application	Title
mouse	WB	Front Immunol; Ddb1 Is Essential for the Expansion of CD4+ Helper T Cells by Regulating Cell Cycle Progression and Cell Death.; Authors - Lingtao Yang
	CoIP	iScience; A Multidimensional Characterization of E3 Ubiquitin Ligase and Substrate Interaction Network.; Authors - Di Chen
human	WB	PLoS One; 14-3-3ε mediates the cell fate decision-making pathways in response of hepatocellular carcinoma to Bleomycin-induced DNA damage.; Authors - Tang Siwei S
human	WB	Virology; DDB1 is a cellular substrate of NS3/4A protease and required for hepatitis C virus replication.; Authors - Kang Xi X ; KD Validated
human	WB	Cell Death Dis; Cul4 E3 ubiquitin ligase regulates ovarian cancer drug resistance by targeting the antiapoptotic protein BIRC3.; Authors - Xiaoyan Hu ; KD Validated
mouse		Cell Rep; SENP3 and USP7 regulate Polycomb-rixosome interactions and silencing functions; Authors - Haining Zhou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sarah (Verified Customer) (07-03-2019)

Total cell lysate (15 ug) was resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk/0.1% Tween-20) for 1h. Membrane was incubated with anti-DDB1 in blocking buffer (1:1000) at 4C overnight. After washing, membrane was incubated in anti-rabbit-HRP in blocking bufffer (1:3000) for 1h at room temperature. Protein was detected using ECL reagent and imaged on a chemiluminescence detection system.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U251/Jurkat

DDB1 Antibody Western Blot, validation (1:1000 dilution) in U251/Jurkat (Cat no:11380-1-AP)