Anticorps Polyclonal de lapin anti-DCUN1D5

• Phare
• Validé par KD/KO

DCUN1D5 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 14810-1-AP

Synonymes

DCN1 like protein 5, DCUN1D5

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Galerie de données de validation

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  WB analysis of HeLa using 14810-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14810-1-AP (DCUN1D5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14810-1-AP

  WB result of DCUN1D5 antibody (14810-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCUN1D5 transfected HEK-293 cells.

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  WB analysis of mouse brain using 14810-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14810-1-AP (DCUN1D5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 14810-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 14810-1-AP (DCUN1D5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14810-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14810-1-AP (DCUN1D5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 14810-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 14810-1-AP (DCUN1D5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14810-1-AP

  IP result of anti-DCUN1D5 (IP:14810-1-AP, 3ug; Detection:14810-1-AP 1:800) with HeLa cells lysate 1840ug.

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  IF Staining of HeLa using 14810-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14810-1-AP (DCUN1D5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules U-937, tissu cérébral de souris, tissu splénique de rat
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

14810-1-AP cible DCUN1D5 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DCUN1D5 Protéine recombinante Ag6534
• Nom complet: DCN1, defective in cullin neddylation 1, domain containing 5 (S. cerevisiae)
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC004169
• Symbole du gène: DCUN1D5
• Identification du gène (NCBI): 84259
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Conjugation of Nedd8 to a cullin protein, termed neddylation, is an evolutionarily conserved process that functions to activate the cullin-RING family E3 ubiquitin ligases, leading to increased proteasomal degradation of a wide range of substrate proteins. Cellular neddylation requires the action of DCN1 (Defective in cullin neddylation protein 1), which, in humans, consists of five homologues designated as DCNL1-5. DCUN1D5 gene encodes the 28 kDa DCUN1 domain-containing protein 5 containing a C-terminal potentiating neddylation domain. The cellular function of DCUN1D5 needs to be explored, recently, a mono-ubiquitination-mediated mechanism that governs nuclear-cytoplasmic trafficking of hDCNL1, was reported to regulate hDCNL1-dependent activation of the cullin-RING E3 ubiquitin ligases in selected cellular compartments.

Publications

Species	Application	Title
human	WB	Elife; SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function.; Authors - Kate E Coleman
human	WB,IHC	Asian Pac J Cancer Prev; In vitro biological characterization of DCUN1D5 in DNA damage response.; Authors - Guo Wei W