Anticorps Polyclonal de lapin anti-DCT

DCT Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 13095-1-AP

Synonymes

DCT, DT, L dopachrome Delta isomerase, L dopachrome tautomerase, TRP 2, TRP2, Tyrosinase related protein 2, TYRP2

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Galerie de données de validation

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  WB analysis of HL-60 using 13095-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 13095-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13095-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 13095-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 13095-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skin using 13095-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 13095-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 13095-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 13095-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HL-60 using 13095-1-AP

  IP result of anti-DCT (IP:13095-1-AP, 4ug; Detection:13095-1-AP 1:500) with HL-60 cells lysate 4000ug.

• 

  IHC staining of human skin using 13095-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13095-1-AP (DCT antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 13095-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13095-1-AP (DCT antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of A549 using 13095-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using DCT antibody (13095-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules A375, cellules A431, cellules COS-7, cellules MCF-7, tissu cutané de rat
• Résultats positifs en IP: cellules HL-60
• Résultats positifs en IHC: tissu cutané humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

13095-1-AP cible DCT dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DCT Protéine recombinante Ag3714
• Nom complet: dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)
• Masse moléculaire calculée: 519 aa, 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC028311
• Symbole du gène: DCT
• Identification du gène (NCBI): 1638
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DCT(L-dopachrome Delta-isomerase) is also named as TYRP2,TRP2 and belongs to the tyrosinase family.human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region(PMID:8206391).

Publications

Species	Application	Title
mouse	IF	Curr Biol; Neuropsin (OPN5) Mediates Local Light-Dependent Induction of Circadian Clock Genes and Circadian Photoentrainment in Exposed Murine Skin.; Authors - Ethan D Buhr
human	IHC	Hum Mol Genet; Transcriptome analysis and molecular signature of human retinal pigment epithelium.; Authors - Strunnikova N V NV
human	WB	J Invest Dermatol; miR-137 inhibits the invasion of melanoma cells through downregulation of multiple oncogenic target genes.; Authors - Chonglin Luo
human	WB	J Cell Mol Med; Kaempferol promotes melanogenesis and reduces oxidative stress in PIG1 normal human skin melanocytes; Authors - Yihui Xie
human	WB	Sci Rep; Loss of tumorigenic potential upon transdifferentiation from keratinocytic into melanocytic lineage.; Authors - Sabrina Fehrenbach
mouse	WB	Pharmaceutics; Anti-Melanogenic Mechanism of Tetrahydrocurcumin and Enhancing Its Topical Delivery Efficacy Using a Lecithin-Based Nanoemulsion.; Authors - Xudong Tang