Anticorps Polyclonal de lapin anti-DCP1A

DCP1A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (2)

N° de cat : 22373-1-AP

Synonymes

Smad4-interacting transcriptional co-activator, Nbla00360, mRNA-decapping enzyme 1A, mRNA decapping enzyme 1A, HSA275986

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Galerie de données de validation

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  WB analysis using 22373-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22373-1-AP (DCP1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 22373-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22373-1-AP (DCP1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 22373-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 22373-1-AP (DCP1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 22373-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22373-1-AP (DCP1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22373-1-AP

  IP result of anti-DCP1A (IP:22373-1-AP, 4ug; Detection:22373-1-AP 1:3000) with mouse brain tissue lysate 3200ug.

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  IHC staining of mouse lung using 22373-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 22373-1-AP (DCP1A Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse lung using 22373-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 22373-1-AP (DCP1A Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 22373-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DCP1A antibody (22373-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 22373-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DCP1A antibody (22373-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HeLa using 22373-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DCP1A (22373-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Jurkat, cellules MCF-7, tissu cérébral de rat, tissu cérébral de souris, tissu pulmonaire de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

22373-1-AP cible DCP1A dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DCP1A Protéine recombinante Ag18027
• Nom complet: DCP1 decapping enzyme homolog A (S. cerevisiae)
• Masse moléculaire calculée: 582 aa, 63 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC007439
• Symbole du gène: DCP1A
• Identification du gène (NCBI): 55802
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DCP1 decapping enzyme homolog A (S. cerevisiae), known as mRNA-decapping enzyme 1A (DCP1A),is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1. This antibody specifically react with the 70kd DCP1A protein.

Publications

Species	Application	Title
human	WB,IF	FEBS Lett; Gemin5 plays a role in unassembled-U1 snRNA disposal in SMN-deficient cells.; Authors - Dongxu Jiang
human	WB,IF	Cell Rep; DIAPH3 condensates formed by liquid-liquid phase separation act as a regulatory hub for stress-induced actin cytoskeleton remodeling; Authors - Ke Zhang
mouse	IF,IP	Cell Rep; The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation; Authors - Nicolas Curdy
human	WB	J Thorac Dis; LncRNA HOXD-AS2 regulates miR-3681-5p/DCP1A axis to promote the progression of non-small cell lung cancer; Authors - Yuanyuan Zhang
human	IF,WB	J Cell Biol; DDX6 modulates P-body and stress granule assembly, composition, and docking; Authors - Nina Ripin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Laurens (Verified Customer) (02-02-2024)

Works well for ICC with bright stain and no background, shown with green channel in image.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: A549 cells, U2OS cells, HEK293 cells

DCP1A Antibody Immunofluorescence validation (1:500 dilution) in A549 cells, U2OS cells, HEK293 cells (Cat no:22373-1-AP)

FH

Tatyana (Verified Customer) (12-04-2023)

Detects P-bodies in ICC, good signal

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human / HeLa cells

DCP1A Antibody Immunofluorescence validation (1:1000 dilution) in Human / HeLa cells (Cat no:22373-1-AP)