Anticorps Polyclonal de lapin anti-DBR1

• Phare
• Validé par KD/KO

DBR1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (2)

N° de cat : 16019-1-AP

Synonymes

DBR1, Lariat debranching enzyme

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Galerie de données de validation

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  WB analysis of HeLa using 16019-1-AP

  WB result of DBR1 antibody (16019-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DBR1 transfected HeLa cells.

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  WB analysis of human brain using 16019-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16019-1-AP (DBR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16019-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16019-1-AP (DBR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16019-1-AP

  IP result of anti-DBR1 (IP:16019-1-AP, 4ug; Detection:16019-1-AP 1:800) with HeLa cells lysate 1560 ug.

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  IP experiment of mouse brain using 16019-1-AP

  IP result of anti-DBR1 (IP:16019-1-AP, 4ug; Detection:16019-1-AP 1:500) with mouse brain tissue lysate 1280 ug.

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  IHC staining of human placenta using 16019-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16019-1-AP (DBR1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 16019-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16019-1-AP (DBR1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 16019-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DBR1 antibody (16019-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules HeLa, cellules HepG2
• Résultats positifs en IP: tissu cérébral de souris, cellules HeLa
• Résultats positifs en IHC: tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

16019-1-AP cible DBR1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DBR1 Protéine recombinante Ag8830
• Nom complet: debranching enzyme homolog 1 (S. cerevisiae)
• Masse moléculaire calculée: 544 aa, 62 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC009472
• Symbole du gène: DBR1
• Identification du gène (NCBI): 51163
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DBR1(Lariat debranching enzyme) hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules. DBR1 belongs to the lariat debranching enzyme family. Inhibiton of Dbr1 can suppress TDP-43 toxicity in primary neurons, suggesting that Dbr1 could be a potential therapeutic target for ALS and related TDP-43 proteinopathies (PMID: 23104007). This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Nat Genet; Inhibition of RNA lariat debranching enzyme suppresses TDP-43 toxicity in ALS disease models.; Authors - Armakola Maria M ; KD Validated
human	WB	Cell; Inborn Errors of RNA Lariat Metabolism in Humans with Brainstem Viral Infection.; Authors - Shen-Ying Zhang
human	WB	Mol Cell Proteomics; Cell cycle regulation of microtubule interactomes: multi-layered regulation is critical for the interphase/mitosis transition.; Authors - Syred Heather M HM
human	IF	PLoS One; Inhibition of U4 snRNA in Human Cells Causes the Stable Retention of Polyadenylated Pre-mRNA in the Nucleus.; Authors - Anne Hett
human	WB, IHC	Int J Mol Sci; Identification of the specific interactors of the human lariat RNA debranching enzyme 1 protein.; Authors - So Masaki
human	IHC	Technol Cancer Res Treat; The Role of DBR1 as a Candidate Prognosis Biomarker in Esophageal Squamous Cell Carcinoma.; Authors - Xiaoqin Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Marta (Verified Customer) (02-18-2020)

Single band in the WB at the expected size, incubated overnight at 4C with a 1/1200 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1200
• Cell Tissue Type: PBMC, B-EBV cells

DBR1 Antibody Western Blot, validation (1/1200 dilution) in PBMC, B-EBV cells (Cat no:16019-1-AP)

FH

Aiswarya (Verified Customer) (12-23-2019)

The Dbr1 antibody was used to check knock down of Dbr1 and worked well. Additional non specific bands come up but do not interfere in the specific size range.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HAP1, HEK293