Anticorps Polyclonal de lapin anti-HIP55

• Phare
• Validé par KD/KO

HIP55 Polyclonal Antibody for WB, IP, IF, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 13015-1-AP

Synonymes

ABP1, Cervical SH3P7, CMAP, DBNL, Drebrin F, drebrin like, Drebrin like protein, HIP 55, SH3P7

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Galerie de données de validation

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  WB analysis of mouse brain using 13015-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 13015-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13015-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13015-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 13015-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13015-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13015-1-AP

  IP result of anti-HIP55 (IP:13015-1-AP, 4ug; Detection:13015-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

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  IHC staining of human breast cancer using 13015-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13015-1-AP (DBNL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13015-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13015-1-AP (DBNL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 13015-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HIP55 antibody (13015-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HepG2 using 13015-1-AP

  Immunofluorescent analysis of HepG2 cells, using DBNL antibody 13015-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green).

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  FC experiment of HepG2 using 13015-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug HIP55 Polyclonal antibody (13015-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, cellules Jurkat, cellules K-562, tissu cardiaque de souris, tissu cardiaque humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Informations sur le produit

13015-1-AP cible HIP55 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HIP55 Protéine recombinante Ag4078
• Nom complet: drebrin-like
• Masse moléculaire calculée: 430 aa, 48 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC031687
• Symbole du gène: HIP55
• Identification du gène (NCBI): 28988
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HIP55, also known as DBNL, ABP1, SH3P7, belongs to the ABP1 family. HIP55 plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. HIP55 acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes (PMID: 14729663). The N terminus of HIP55 contains a putative actin-binding domain found in drebrins, which are involved in brain development, and the C terminus contains an SH3 domain. Expect a band 55 kDa in size corresponding to HIP55 by western blotting.

Publications

Species	Application	Title
human,mouse	WB,IP	Acta Pharmacol Sin; The multifunctional adaptor protein HIP-55 couples Smad7 to accelerate TGF-β type I receptor degradation.; Authors - Yang Sun ; KD Validated
mouse	WB,IF,IP	J Neurosci; Drebrin-like (Dbnl) controls neuronal migration via regulating N-cadherin expression in the developing cerebral cortex.; Authors - Seika Inoue
		J Cell Sci; VEGF-A stimulates podosome-mediated collagen-IV proteolysis in microvascular endothelial cells.; Authors - Thomas Daubon
mouse	WB	J Microbiol; Effect of Salmonella treatment on an implanted tumor (CT26) in a mouse model.; Authors - Yun Misun M
human	IF	Nature; A cross-species proteomic map reveals neoteny of human synapse development; Authors - Li Wang